六经头痛片镇痛作用特点与比较优势研究

Study of action characteristics and comparative advantage of Liujing Toutong Tablets about analgesia

目的通过不同镇痛药效学模型、实验性偏头痛模型及气滞血瘀模型,与同类药正天丸对比,探讨六经头痛片的镇痛作用特点及优势。方法通过小鼠热板实验、醋酸扭体实验,观察六经头痛片对温热刺激、化学刺激引起疼痛的影响;采用硝酸甘油诱发实验性大鼠偏头痛模型,观察其对搔头反应潜伏期及搔头次数的影响,检测大鼠血清一氧化氮(NO)、一氧化氮合酶(NOS)及β-内啡肽(β-EP)水平;采用sc盐酸肾上腺素联合冰水浴的方法制备气滞血瘀模型,观察其对脑血流速率及血液流变学指标的影响。结果六经头痛片可显著增加热板实验小鼠痛阈值,减少醋酸引起的小鼠扭体次数;显著延长偏头痛模型大鼠搔头反应的潜伏期,减少搔头次数,显著降低偏头痛大鼠血清NO、NOS水平,升高β-EP水平;可显著升高气滞血瘀模型大鼠脑血流速率,降低全血黏度及血浆黏度。结论六经头痛片对中枢疼痛、炎性疼痛及实验性偏头痛模型具有显著的镇痛作用,对气滞血瘀模型具有显著的活血化瘀作用,具有起效早,药效持续时间长的特点。与同类药正天丸比较,镇痛作用相当,但起效快,多靶点。

Objective To observe the action characteristics and comparative advantage of Liujing Toutong Tablets（LTT） about analgesia compared with Zhengtian Pills through different analgesic pharmacodynamic model, experimental migraine model and qi stagnation and blood stasis model. Methods Analgesic effect of LTT on thermal stimulation and chemical stimulation through hot plate test and writhing test caused by acetic acid were observed. Experimental migraine model was induced by nitroglycerin, effect of LTT on the latency and times of scratching head response of migraine model rats were observed, and levels of NO, NOS, and β-EP in serum of migraine model rats were detected. The qi stagnation and blood stasis model was prepared by sc epinephrine hydrochloride combined with ice-water bath, and effect of LTT on cerebral blood velocity and hemorheology of qi stagnation and blood stasis model rats was observed. Results LTT could significantly prolong the latency of hot plate test and decrease writhing times caused by acetic acid in mice. LTT could significantly prolong the latency and decrease the times of scratching head response of migraine model rats, LTT could significantly decrease the level of NO and NOS, increase β-EP obviously in serum of migraine model rats. Also LTT could significantly increase the cerebral blood velocity and decrease blood viscosity as well as plasma viscosity of qi stagnation and blood stasis model rats. Conclusion LTT has significant analgesia effect with characteristics of working early and lasting long on central pain, inflammatory pain and experimental migraine model, also has significant activating blood and dissolving stasis effect on qi stagnation and blood stasis model. The analgesia effect of LTT is equal to Zhengtian Pills, but LTT works early and has multi-target compared with Zhengtian Pills.