鲍温病组织中HPV感染与微卫星DNA改变的关系

Relationship between HPV infection and changes of microsatellite DNA in tissues of Bowen′s disease

目的分析鲍温病(BD)组织中人乳头瘤病毒(HPV)感染与微卫星(MS)DNA改变的关系。方法收集2011年1月-2015年10月医院收治的30例BD患者的病理组织标本,作为观察组,选取同期外科切除的正常组织标本30份作为对照组,均采用基因芯片技术检测HPV分型,探讨BD组织HPV感染与微卫星DNA改变的关系。结果观察组HPV感染阳性11例,感染率为36.67%,明显高于对照组(P<0.05),且观察组检出HPV亚型均为高危型,其中HPV16亚型感染所占比例最高,为63.64%;观察组MS DNA改变检出率为20.00%,明显高于对照组(P<0.05),其LOH所占比例高于对照组(P<0.05);BD组织与P53连锁的D17S796、D17S926位点共发生LOH 3例,与错配修复基因hMLH1连锁的D3S1612、D3S1029位点发现MSI 1例,LOH 2例;HPV感染11例中,MS DNA改变5例占45.45%,其中HPV16亚型感染中MS DNA改变中MSI占14.29%,LOH占42.36%;HPV58亚型感染中LOH占100.00%;HPV阴性中LOH 1例,占5.26%;HPV阳性组MS DNA改变发生率明显高于HPV阴性组(P<0.05)。结论 BD发生与高危型HPV感染及MS DNA改变有一定相关性,P53基因突变可能参与BD发病过程;高危型HPV感染与BD MS DNA改变有一定的关系。

OBJECTIVE To analyze the relationship between human papilloma virus（HPV）infection and changes of microsatellite（MS）DNA in tissues of Bowen＇s disease（BD）.METHODS The pathological specimens of 30 patients with BD treated in our hospital from Jan.2011 to Oct.2015 were selected as observation group,and 30 normal tissue specimens collected at the same period were taken as control group.The types of HPV were detected by gene chip technique,and the relationship between HPV infection and changes of microsatellite DNA in tissues of BD was discussed.RESULTS There were 11 cases of positive HPV infection in observation group,and the infection rate was 36.67%,which was significantly higher than that in control group（P〈0.05）.In observation group,the subtypes of HPV were all high-risk types,and the proportion of HPV16 subtype infection was the highest（63.64%）.The detection rate of MS DNA changes in observation group was 20.00%,which was significantly higher than that in control group（P〈0.05）,and the proportion of LOH in observation group was higher than that in control group（P〈0.05）.There were 3 cases of LOH at D17 S796 and D17 S926 loci associated with tissues of BD and P53,and 1 case of MSI and 2 cases of LOH at D3 S1612 and D3 S1029 loci associated with mismatch repair gene hMLH1.Among the 11 cases of HPV infection,there were 5 cases of MS DNA changes accounting for 45.45%,among which MSI in MS DNA changes in HPV16 subtype infection accounted for 14.29% and LOH accounted for 42.36%.In HPV58 subtype infection,LOH accounted for 100.00%.There was 1 case of LOH in negative HPV infection,accounting for 5.26%.The incidence of MS DNA changes in HPV positive group was significantly higher than that in HPV negative group（P〈0.05）.CONCLUSION There are certain correlations between the occurrence of BD and high-risk HPV infection and changes of microsatellite DNA.P53 gene mutation may be involved in the pathogenesis of BD,and there is a certain correlation between high-risk HPV infection and MS DNA changes of BD.