载脂蛋白C3基因rs5128多态性与不同体质指数冠心病患者血脂水平及冠状动脉狭窄程度的相关性

Relationship between the ApoC3 rs5128 polymorphism and plasma lipid levels and the severity of coronary stenosis in coronary heart disease patients with different body mass index

目的探讨中国汉族人群载脂蛋白C3(ApoC3)基因rs5128多态性与不同体质指数(BMI)冠心病(CHD)患者血脂水平及冠状动脉狭窄程度的相关性。方法根据BMI将312例CHD患者分为正常体重组(205例)和超重/肥胖组(107例)。收集所有患者的生理生化资料和冠状动脉造影数据,采用Gensini评分法评价CHD患者的冠状动脉狭窄程度。提取外周血白细胞DNA并应用聚合酶链反应-限制性片段长度多态性法对ApoC3rs5128多态性分型。结果超重/肥胖组体重、BMI、高血压患病率、甘油三酯(TG)、脂蛋白a、TG/高密度脂蛋白胆固醇(HDLC)、总胆固醇(TC)/HDLC、低密度脂蛋白胆固醇(LDLC)/HDLC和载脂蛋白B100(ApoB100)/载脂蛋白AI(ApoAⅠ)水平高于正常体重组,HDLC和ApoAⅠ水平低于正常体重组(P<0.05)。在正常体重组中,G等位基因携带者高血压患病率显著高于CC基因型患者(P<0.05);在超重/肥胖组中,G等位基因携带者TG和TG/HDLC水平显著高于CC基因型患者(P<0.05)。在正常体重组和超重/肥胖组中,rs5128多态性基因型和等位基因频率在不同冠状动脉狭窄程度亚组中的分布差异无统计学意义(P>0.05)。结论在超重/肥胖CHD患者中,rs5128多态性G等位基因与血浆TG和TG/HDLC水平升高显著相关,但与冠状动脉狭窄程度无明显关联。

Aim The aim of the study was to investigate the relationship between the ApoC3 rs5128 polymor- phism and plasma lipid levels and the severity of coronary stenosis in the coronary heart disease （CHD） patients with differ- ent body mass index （BM[） in Chinese Han population. Methods Three hundred and twelve CHD patients were di- vided into healthy weight group （205 patients） and overweight/obese group （ 107 patients）. Physiological, biochemical and coronary angiographical data were collected for all patients. The severity of coronary stenosis was assessed by the Gensini scoring system. Genome DNA was extracted from peripheral blood leucocytes and the genotypes of the ApoC3 rs5128 polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism method. Results The overweight/obese patients had higher weight, BMI, prevalence of hypertension, triglyeeride （ TG）, lipopro- tein （a）, TG/high-density lipoprotein cholesterol （HDLC）, total cholesterol （TC）/HDLC, low-density lipoprotein choles- terol （LDLC）/HDLC and apollpoprotein B 100 （ApoB100）/apolipoprotein AI （ApoA I ）, and lower HDLC and ApoA I than the patients with healthy weight （P〈0.05）. In healthy weight group, the G carriers had higher prevalence of hyper- tension than the patients with the CC genotype （P〈0.05）. In overweight/obese group, the G carriers had higher levels of TG and TG/HDLC than the patients with the CC genotype （P 〈 0.05 ）. In healthy weight group and overweight/obese group, there were no significant differences in the distributions of the genotypes or alleles of the rs5128 polymorphism a- mong the tertiles of the Gensini scores. Conclusion The ApoC3 rs5128 polymorphism is significantly associated with plasma TG and TG/HDLC levels, but not with the severity of CHD.