9种生物制剂对甘薯长喙壳菌的抑制作用及防治效果

Inhibitory Activities and Control Efficacy of Nine Biopesticides to Ceratocystis fimbriata Ellis et Halsted

为筛选防治甘薯黑斑病的生物农药,测定了9种生物制剂对甘薯长喙壳菌孢子萌发和菌丝生长的抑制作用,并进行了防治效果验证。结果表明,四霉素对分生孢子萌发以及菌丝生长均表现出最强的抑制作用,EC50分别为0.10和0.15 mg/L;丁子香酚次之,EC50分别为0.24和0.93 mg/L。四霉素对薯苗黑斑病具有较好的防治效果,当接种甘薯长喙壳菌分生孢子浓度为5×104孢子/m L时,四霉素有效用量6与9 mg/L处理的防治效果分别为66.67%和78.57%;当接种浓度为5×105孢子/m L时,四霉素有效用量6与9 mg/L处理的防治效果分别为77.44%和90.23%。本研究为生物农药应用于甘薯黑斑病的防治提供了依据。

To screen biopesticides for controlling black rot of sweet potato, the inhibitory activities of 9 biopesticides against mycelial growth and conidial germination of Ceratocystis fimbriata Ellis et Halsted were measured and the control efficacies were tested. The results showed that tetramycin had the highest inhibitory activities to conidial germination and mycelial growth, with the EC50 values of 0.10 and 0.15 mg/L, respectively. Eugenol showed the second highest inhibitory activities, with the EC50 values of 0.24 and 0.93 mg/L, respectively. When inoculated C. fimbriata with 5×104 spores/m L, control efficacy of tetramycin at 6 and 9 mg/L were 66.67% and 78.57%, respectively; At 5×105 spores/m L, the efficacy were 77.44% and 90.23%, respectively. This study provided an experimental basis for rational use of biopesticides for controlling black rot of sweet potato.