不同推拿手法对食蟹猴轻度颈动脉粥样硬化模型的影响

The influence of massage manipulation on early carotid artery atherosclerotic plaque model of cynomolgus monkeys

目的探讨不同颈椎推拿手法对食蟹猴轻度颈动脉粥样硬化(CAS)模型稳定性的影响。方法将18只食蟹猴建立轻度CAS模型,并以高脂饲料喂养8周,用彩超证实其造模成功。再将18只食蟹猴随机分为3组(每组6只),分别是:颈椎旋转组、推桥弓组和模型对照组。分别对颈椎旋转组和推桥弓组的食蟹猴进行相应的手法干预,共干预30 d,模型对照组不做特殊干预。最后将动物麻醉后处死,取颈总动脉分别进行HE染色、拉曼实验和压力实验。结果 HE染色可见颈总动脉血管内壁损伤严重,有斑块形成。拉曼试验中,3组均有明显的1450及1660脂质特征峰,但三组特征峰的相对强度差异无显著性意义(P>0.05)。压力实验中,三组中食蟹猴颈动脉斑块破裂压力检测结果显示无显著性意义(P>0.05)。结论在食蟹猴轻度CAS病变中,短期内施用颈椎旋转手法和推桥弓手法并不会改变斑块的稳定性。

Objective To explore the influence of different cervical massage manipulation on the stability of early carotid artery atherosclerosis（CAS） model of cynomolgus monkeys. Methods 18 cynomolgus monkeys were developed early CAS model, and were fed with a high-fat diet for 8 weeks. The success of model was confirmed by Color Doppler Ultrasound. Then all monkeys were divided into three groups randomly： the Cervical Rotatory Manipulation（CRM） group, the Manipulation of Pushing on Qiaogong（MPQ） group and the control model group. The animals of the CRM group and the MPQ group underwent relevant interventions respectively for 30 days, but the control model group was not given any intervention.Finally all animals were euthanized, and the common carotid artery underwent HE staining, Raman test,pressure test. Results HE staining showed that the intima of common carotid artery were severely injured,and there was formation of CAS plaques. In Raman test, lipid characteristic peaks on 1450 and 1660 were obvious in 3 groups. However, there was no significant difference on the relative intensity of the 3 groups（P〈0.05）. In pressure test, there was no significant difference on the results of fracture pressure of CAS plaques in3 groups（P〈0.05）. Conclusion In the early stage of CAS in cynomolgus monkeys, the application of CRM and MPQ in short term have no impact on the stability of plaques.