甘蓝型油菜甘油三磷酸脱氢酶(BnaGPDH)基因的克隆与表达

Molecular cloning and expression profile of Bna GPDH gene in Brassica napus

甘油三磷酸脱氢酶(GPDH)是三酰甘油(TAG)合成的关键酶。为了阐明甘蓝型油菜(Brassica napus)GPDH(BnaGPDH)的表达特性,本研究采用反转录PCR(RT-PCR)方法,获得BnaGPDH基因的3条全长编码序列(CDS),分别命名为BnaGPDH1、BnaGPDH2、BnaGPDH3,长度分别为1395、1395、1389 bp。生物信息学分析表明,BnaGPDHs具有GPDH基因家族的特征,BnaGPDHs与其他植物GPDHs有高度同源性。时空表达分析表明,BnaGPDHs是组成型表达基因,在花中的表达量最高,叶中的表达量最低。BnaGPDHs在幼嫩种子发育的第5周表达量普遍提高,在果荚发育到第5周表达量明显降低。逆境分析表明,BnaGPDHs响应多种逆境胁迫,可能与植物抗逆机制有关。

Glycerol-3-phosphate dehydrogenase（GPDH） is a key enzyme in triacylglycerol（TAG） biosynthesis. In order to elucidate the expression feature of BnaGPDHs in Brassica napus, three GPDH full-length coding sequences（1 395, 1 395 and 1 389 bp） were obtained by reverse transcription PCR, and designated as BnaGPDH1, BnaGPDH2 and BnaGPDH3, respectively. Bioinformatic analysis shows that they have the characteristics of the GPDH family, and amino acid sequences of BnaGPDHs are highly homologous to GPDHs in other plants. Spatio-temporal expression results show that BnaGPDHs are genes of constitutive expression, with the highest expression in flower and the lowest expression in leaf. BnaGPDH expression levels were generally increased in developing seed at 35 days after flowering, at the same time they were significantly decreased in developing capsule. BnaGPDHs responded to abiotic stresses, indicating they might be concerned with the mechanism of plant stress resistance.