Twist在食管鳞状细胞癌中的表达与临床预后的关系

Correlation of Twist expression in esophageal squamous cell carcinomas with clinical prognosis

目的观察Twist在食管鳞状细胞癌(鳞癌)患者中的表达情况,分析Twist阳性表达与食管鳞癌患者脉管内出现瘤栓、无病生存期的关系,为减少肿瘤复发、延长无病生存时间、改善预后提供临床证据。方法回顾性分析2010年6月至2012年6月四川省肿瘤医院胸外科70例食管鳞癌并接受手术治疗、完成临床随访患者的临床资料,其中男39例、女31例,平均年龄63.6岁。对其术后石蜡标本进行免疫组化染色,观察Twist在正常食管组织、肿瘤组织和脉管瘤栓中的表达情况。结果 Twist阳性表达率在正常食管组织中和肿瘤组织中分别为42.9%、77.1%,其差异有统计学意义(P<0.05)。脉管瘤栓阳性和阴性的食管鳞癌患者肿瘤细胞中Twist阳性表达率分别为74.3%、80.0%,差异无统计学意义(P>0.05)。Twist在肿瘤细胞和在脉管瘤栓内阳性表达率分别为74.3%、71.4%,差异无统计学意义(P>0.05)。Twist在脉管瘤栓阳性患者的淋巴管内和血管内表达分别为56.0%、72.0%,差异无统计学意义(P>0.05)。结论 Twist在食管癌肿瘤组织中的表达明显高于正常组织,但肿瘤细胞内Twist是否阳性表达与该类患者平均无病生存期长短的差异无统计学意义(P>0.05),目前尚不能认为Twist的表达情况可以作为判断食管癌预后的参考指标之一,有待进一步研究。

Objective To observe the expression of Twist in esophageal squamous cell carcinoma （ESCC） and analyze the relationship between positive expression of Twist and disease-free survival, and to provide clinical evidence for reducing tumor recurrence, prolonging disease-free survival and improving prognosis. Methods Retrospective analysis of 70 ESCC patients receiving thoracic surgery from June 2010 to June 2012 in the Department of Thoracic Surgery, Sichuan Cancer Hospital was done, including 39 males and 31 females with an average age of 63.6 years. The expression of Twist in normal esophageal tissue, tumor tissue and vascular tumor emboli was observed by immunohistochemical staining of paraffin specimens. Results The positive rate of Twist in normal esophageal tissues was 42.9%, and in tumor tissue was 77.1% （P〈0.05）. The positive expression rate of Twist in tumor cells was 74.3% in patients with vascular tumor emboli and 80.0% in patients without vascular tumor emboli （P〉0.05）. The positive expression rate of Twist in tumor cells and in vascular tumor emboli was 74.3% and 71.4%, respectively （P〉0.05）. The expression of Twist in lymphatic vessels and blood vessels of patients with vascular tumor emboli was 56.0% and 72.0%, respectively （P〉0.05）. Conclusion Twist expression in esophageal cancer tissues is significantly higher than that in normal tissues, but there is no significant difference in the positive expression of Twist between tumor cells and the mean disease-free survival （P〉0.05）. At present, Twist expression can not be used as a prognostic indicator of esophageal cancer, and more researches need be further implemented.