我国部分地区牛源金黄色葡萄球菌基因多态性分型研究

Identification of genetic polymorphism of Staphylococcus aureus isolated from bovines mastitis in parts of China

为了解我国牛源金黄色葡萄球菌(S.aureus)的基因多态性,本研究利用随机引物多态性扩增(RAPD)体系对174株分离自贵州、内蒙古、四川、上海、甘肃等地奶牛乳房炎的S.aureus及一株标准菌CVCC2246进行基因分型。结果表明,175株S.aureus均得到清晰的RAPD指纹图谱,扩增产物为1~9个片段,产物大小为240 bp^4 500 bp。所有菌株共分为8个基因型,其中1型16株;2型和3型各37株;4型15株;5型18株;6型14株;7型13株;8型8株。2型和3型菌株占总菌株42%以上,在贵州、内蒙古、四川、上海、甘肃分布广泛,为流行优势基因型;但5型是内蒙古地区的流行优势基因型。各地区菌株基因型比例有明显差异,可能与奶牛饲养水平和环境差异有关。

In order to-know the gene polymorphisms of dairy sourced Staphylococcus aureus, 174 strains S.aureus isolated from dairy cows with mastitis in Guizhou, Inner Mongolia, Sichuan, Shanghai, Gansu and a standard strain were analyzed by random amplified polymorphic DNA （RAPD） in this study. The result showed that the amplified products bands were 1 to 9 fragments and the product sizes was 240 bp to 4 500 bp. Genotyping of these isolates indicated that all isolates were distributed into 8 different genotype. Among 175 isolates, 16 were type 1, 37 were type 2 and type 3 respectively, 15 were type 4, 18 were type 5, 14 were type 6, 13 were type 7 and type 8, 8 were type 8. Both of type 2 and type 3 were the dominant genotypes which were widely distributed in Guizhou, Shanghai, Sichuan, and Gansu provinces. Type 5 was the dominant genotype in Inner Mongolia. The proportion of 8 genotypes in different places were significant diffrence, which could be related to the feeding level and environment difference of dairy cow.