针灸对气虚血瘀型糖尿病大鼠模型下肢血管病变的影响

Effect of Acupuncture and Moxibustion on Vascular Lesions of Lower Limbs in Diabetic Rats with Qi Deficiency and Blood Stasis Syndrome

目的:探讨针灸疗法对气虚血瘀证2型糖尿病大鼠下肢血管病变的影响。方法:将35只雄性SD大鼠分为对照组7只、模型组14只和针灸组14只。通过高脂喂养、大黄灌胃联合多次尾静脉注射小剂量链脲佐菌素(STZ)的方法建立气虚血瘀型2型糖尿病大鼠模型,针灸胰俞穴、足三里进行干预,动态观察针灸对2型糖尿病大鼠血清氧化低密度脂蛋白(Ox-LDL)和股动脉血凝素样氧化低密度脂蛋白受体LOX-1表达的影响。结果:与对照组比较,模型组大鼠血清Ox-LDL水平和LOX-1蛋白表达明显升高(P<0.05),与模型组比较,针灸组大鼠血清Ox-LDL水平和LOX-1蛋白表达显著降低(P<0.05)。结论:2型糖尿病大鼠血清Ox-LDL显著升高,提示血管病变的发生,针灸干预可通过降低LOX-1蛋白表达抑制2型糖尿病大鼠血管病变。

Objective To investigate the effect of acupuncture on lower extremity vascular disease in type 2 diabetic rats with Qi defi- ciency and blood stasis syndrome. Methods 35 male SD rats were divided into two groups ： control group of 7, model group of 14 and acu- puncture group of 14. The high fat diet, rhubarb combined with multiple intravenous injection of a small dose of streptozotocin （STZ） of qi deficiency and blood stasis rat model of type 2 diabetes, Yu Xue, Zusanli acupuncture pancreas intervention, dynamic observation of acu- puncture effect on the expression of LOX - 1 in serum of Ox - LDL vein and femora/in type 2 diabetic rats. Reslflts Compared with the con- trol group, the expression of model group rats serum levels of Ox - LDL and LOX - 1 protein increased significantly （P 〈 0. 05 ）, compared with the model group, the expression of acupuncture group rats serum levels of Ox - LDL and LOX - 1 protein decreased significantly （P 〈 0. 05 ）. Conclusion The serum Ox - LDL levels in type 2 diabetic rats increased significantly, suggesting the occurrence of vasculopathy, and acupuncture intervention could inhibit the vascular lesion in type 2 diabetic rats by decreasing the expression of LOX - 1 protein.