摘要
目的:建立同时测定干髓糊剂中丁香酚和麝香草酚含量的方法。方法:采用高效液相色谱法。色谱柱为Wonda Cract C_(18),流动相为甲醇-0.2%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为274 nm,柱温为25℃,进样量为10μL。结果:丁香酚和麝香草酚检测质量浓度线性范围分别为0.02~0.19 mg/mL(r=0.999 7)、0.03~0.30 mg/mL(r=0.999 6);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为95.85%~99.84%(RSD=1.11%,n=9)、95.81%~100.91%(RSD=1.59%,n=9)。结论:该方法操作简便、结果准确,可用于干髓糊剂中丁香酚和麝香草酚含量的同时测定。
OBJECTIVE:To establish a method for simultaneous determination of eugenol and thymol in mummification pastes. METHODS:HPLC method was adopted. The determination was performed on Wonda Cract C_(18) column with mobile phase consisted of methanol-0.2% phosphoric acid(gradient elution)at the flow rate of 1.0 mL/min. The detection wavelength was 274 nm,and column temperature was set at 25 ℃. The sample size was 10 μL. RESULTS:The linear ranges of eugenol and thymol were 0.02-0.19 mg/mL(r=0.999 7)and 0.03-0.30 mg/mL(r=0.999 6). RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 95.85%-99.84%(RSD=1.11%,n=9)and 95.81%-100.91%(RSD=1.59%,n=9). CONCLUSIONS:The method is simple and accurate,and can be applied to simultaneous determination of eugenol and thymol in mummification pastes.
作者
曹伟宇
贺文娟
雷丸
王晓娟
CAO Weiyu HE Wenjuan LEI Wan WANG Xiaojuan(State Key Lab of Military Stomatology/National Clinical Research Center for Oral Diseases/Shaanxi Engineering Research Center for Dental Materials and Advanced Man- ufacture/Dept, of Pharmacy, Stomatology Hospital of Fourth Military Medical University, Xi'an 710032, China)
出处
《中国药房》
CAS
北大核心
2017年第30期4268-4271,共4页
China Pharmacy
基金
军队医疗机构制剂标准提高研究项目(No.13ZJZ08)