M型钾离子通道开放剂对心肺复苏大鼠心肌的保护作用

Protective effect of M type potassium channel opener in myocardium of cardiopulmonary resuscitation rats

目的探讨M型钾离子通道开放剂对心肺复苏大鼠心肌的保护作用。方法将健康Wistar大鼠60只采用随机数字表法分为假手术组(A组)、心脏骤停复苏组(B组)、M型钾离子通道开放剂干预组(C组)各20只,按心肺复苏后自主循环恢复6 h、12 h、24 h、48 h、72 h,每组分别处死大鼠4只,采集心肌标本,检测心肌指标(超氧化物歧化酶、丙二醛、心肌肌钙蛋白T),并观察心脏结构(左心室容积、心室间隔厚度)。结果组间比较:B组在所有时点超氧化物歧化酶较A组降低,C组较B组升高,差异均有统计学意义(P<0.05);组内比较:B组和C组在12 h、24 h逐渐下降,48 h和72 h逐渐升高,差异均有统计学意义(P<0.05)。组间比较:B组在所有时点心肌丙二醛、心肌肌钙蛋白T、左心室容积、心室间厚度较A组升高,C组只有24 h心室间厚度高于A组,差异均有统计学意义(P<0.05);组内比较:B组和C组心肌丙二醛、心肌肌钙蛋白、C组的左心室容积在12 h、24 h逐渐升高,48h和72 h逐渐下降,差异均有统计学意义(P<0.05)。结论 M型钾离子通道开放剂可减轻心肺复苏大鼠的心肌损伤,具有良好的心肌保护作用。

Objective To explore the protective effect of M-type potassium channel opening agent on myocardium of cardiopulmonary resuscitation rats.Methods A total of 60 healthy Wistar rats were randomly divided into sham operation group （group A）,cardiac resuscitation group（group B）,M-type potassium channel opener intervention group（group C）,and there were 20 rats in each group.Four rats were sacrificed in each group at 6 h,12 h,24 h,48 h and 72 h after cardiopulmonary resuscitation recovery.The myocardium samples were collected, the myocardial parameters （superoxide dismutase, malondialdehyde, cardiac troponin T ） were detected,and the cardiac structure（left ventricular volume,ventricular septal thickness）were observed.Results Compared with group A,the levels of superoxide dismutase at any time point in group B were all lower,and those in group C were higher than group B ,the difference were significant（P〈0.05）.Both in group B and in group C,the levels of superoxide dismutase at 12 h and 24 h were all decreased gradually,but were increased at 48 h and 72 h,and the difference were significant（P〈0.05）.The levels of malondialdehyde ,cardiac troponin T,left ventricular volume,and ventricular tachycardia at any time point in group B were higher than those in group A,and those in group C were lower than group B.The ventricular septal thickness of group C was thicker than that of group A 24 h after operation.Both in group B and in group C,the levels of malondialdehyde and cardiac troponin T at 12 h and 24 h were increased gradually,but were decreased at 48 h and 72 h.And the left ventricular volume were the same changes in group C. The difference were significant （P〈 0.05 ）.Conclusion M-type potassium channel opener can protect myocardial cells and reduce the impairment on myocardial of cardiopulmonary resuscitation rats.