PD-L1在原发性胆汁性肝硬化患者外周血单个核细胞中的变化及意义

Change of PD-L1 in Peripheral Blood Mononuclear Cells of Patients with Primary Biliary Cirrhosis and Its Significance

目的探究程序性细胞死亡配体1(PD-L1)在原发性胆汁性肝硬化(PBC)患者外周血单个核细胞(PBMC)中的表达情况及意义。方法收集2013年2月～2015年12月于长海医院确诊的23例PBC患者和23例健康对照者外周血,用密度梯度离心法分离出PBMC后进行培养,以实时荧光定量PCR法(qRT-PCR)检测PBMC中PD-L1的mRNA表达状况,以酶联免疫吸附反应(ELISA)法检测培养液中TNF-α,TGF-β,IL-23和IL-17等细胞因子的表达量,两组之间的变量比较采用两独立样本t检验,以Pearson相关系数表示两变量之间的关系。结果实验组与对照组PD-L1的mRNA表达水平为0.23±0.08 vs 1.27±0.40(t=12.23,P<0.000 1),差异具有统计学意义。Pearson相关性分析显示PD-L1与IL-23成负相关(r=-0.531,P=0.009),差异具有统计学意义。结论 PD-L1可能参与了PBC疾病形成过程,是该病预测和治疗潜在的生物标志物。

Objective To explore the expression, of PD-L1 in peripheral blood mononuclear cells （PBMC） from patients with primary biliary cirrhosis （PBC） and its significance. Methods Peripheral blood of 23 healthy individuals and 23 patients with PBC diagnosed in Changhai Hospital was collected during February 2013 to December 2015. PBMC was separated by density gradient centrifugation, then cultured. The mRNA expression of PD-L1 in PBMC was examined by real-time fluorescent quantitative PCR （qRT-PCR）,the expression of cytokines such as TNF-α, TGF-β, IL-23, IL-17 in medium was exam- ined by enzyme-linked immunosorbent assay （ELISA）. Two independent samples＇ t test was used to compare variables in two groups. Pearson correlative coefficient was used to reveal the relationship between two variables. Results mRNA ex- pression of PD-L1 in experiment and control groups was 0.23±0.08 vs 1.27＋0.40 （t=12.23,P〈0. 000 1） and there was statistical significance. Pearson correlation analysis revealed that PD-L1 was negatively correlated with IL-23（r=-0. 531 ,P =0. 009） with difference statistically. Conclusion PD-L1 might involve in the development of PBC,and could be the potential biomarker for predicting and treating this disease.