miR-126在血液透析并发冠心病患者中检测的临床意义

Clinical Significance of Detecting miR-126 in Hemodialysis with Coronary Artery Disease

目的探讨血液透析并发冠心病患者检测miR-126的临床意义。方法血液透析患者60例中并发冠心病组(A组)35例,其中单支病变12例,双支病变13例,多支病变10例。无并发冠心病组(B组)25例。正常对照组(C组)40例。茎环逆转录荧光定量PCR检测miR-126表达水平并进行统计学分析。结果 A组miR-126表达量为0.228±0.023,比C组(0.356±0.027)和B组(0.371±0.022)均低(F=5.32,P<0.5)。其中A组分别与B组和C组比较差异均有统计学意义(t=6.32～8.35,均P<0.05)。单支病变组miR-126表达量为0.246±0.025,双支病变组为0.228±0.021,多支病变组为0.206±0.019,三组比较差异均有统计学意义(F=6.15,均P<0.05)。其中两两比较差异均无统计学意义(t=8.41-11.65,均P<0.05)。结论 miR-126检测对血透患者透析时限预警有无并发冠心病意义重大。

Objective To investigate the clinical significance and level changes of miR-126 on hemodialysis patients. Methods 60 cases from hemodialysis were divided into patients with coronary artery disease （35cases）,including single coronary artery lesion （12cases） and two coronary artery lesion （13cases） and coronary multivessel lesions （10cases）, or no coronary artery disease （25cases）. They were detected miR-126 expression by real-time PCR. Results There were obvious differences of miR-126 expression levels between patients and normal group （F= 5.32,P〈0. 05）,and obvious differences of A groups and B groups, or C groups （t= 6.32- 8.35, all P〈0.05）. Among single coronary artery lesion, two coronary artery lesion, and coronary multivessel lesions, there were obvious differences of miR-126 expression levels （F= 6.15, P〈0.05）. The single coronary artery lesion had a higher miR-126 expression levels than those of two coronary artery lesion and coronary multivessel lesions （t=8.41±11.65,all P〈0.05）. Conclusion Detection of miR-126 expression levels could be related to he- modialysis with coronary artery disease.