多囊卵巢综合征CITED2基因表达变化的临床意义

Clinical significance of CITED2 gene expression in patients with polycystic ovary syndrome

目的:探讨多囊卵巢综合征(polycystic ovary syndrome,PCOS)与CITED2基因表达变化的相关性。方法:收集新疆维吾尔自治区人民医院北院2014年5月至2016年12月收治的PCOS患者114例,另取新疆维吾尔自治区人民医院北院正常月经周期的健康育龄期女性80例作为对照组。使用电化学发光法测定卵泡刺激素(follicle-stimulating hormone,FSH)、黄体生成素(luteotropic hormone,LH)、睾酮(testosterone,T)及雌二醇(estradiol,E2)水平。稳态模型测定胰岛素分泌指数(homeostasis model assessmentβ,HOMA-β)和胰岛素抵抗指数(homeostasis model assessment-insulin resistance,HOMA-IR)。荧光定量PCR检测CITED2基因表达。结果:PCOS患者CITED2基因的2^(-△Ct)值为0.146±0.032,对照组为0.097±0.022,两组间差异具有统计学意义(t=3.135,P=0.032)。CITED2基因表达与PCOS患者FSH呈负相关(r=-0.216,P=0.041),与LH(r=0.382,P=0.022),T(r=0.394,P=0.016),E2(r=0.375,P=0.026),HOMA-β(r=0.351,P=0.031)和HOMA-IR(r=0.326,P=0.033)呈正相关。结论:PCOS患者CITED2基因高表达,这可能是PCOS患者性激素紊乱和胰岛素抵抗的主要影响因素。

Objective： To analyze the correlation between polycystic ovary syndrome（PCOS） and the change of CITED2 gene expression. Methods： A total of 114 PCOS patients in People＇s Hospital of Urumqi Autonomous Region were collected in this study, 80 healthy reproductive aged women with normal menstrual cycle were selected as the control group. The expression of follicle-stimulating hormone（FSH）, luteotropic hormone（LH）, testosterone（T）, and estradiol（E2） was detected by electrochemiluminescence. Homeostasis model assessment β（HOMA-β） and homeostasis model assessment-insulin resistance（HOMA-IR） were determined by the steady state model. CITED2 gene expression was detected by qRT-PCR. Results： The 2^（-△Ct） value of CITED2 gene in PCOS patients was 0.146±0.032, and in the control group was 0.097±0.022. There was significant difference between the two groups（t=3.135, P=0.032）. The expression of CITED2 gene was negatively correlated with FSH（r=-0.216, P=0.041） in patients with PCOS, and positively correlated with LH（r=0.382, P=0.022）, T（r=0.394, P=0.016）, E2（r=0.375, P=0.026）, HOMA-β（r=0.351, P=0.031） and HOMA-IR（r=0.326, P=0.033）. Conclusion： CITED2 gene is highly expressed in patients with PCOS, which may be the main influencing factor of sex hormone disorder and insulin resistance.