STC-1在宫颈癌中的表达及其在预后判断中的价值

Expression of stanniocalcin 1 in cervical cancer and its clinical value in prognosis

目的:研究宫颈癌中分泌蛋白斯坦尼钙调节蛋白1(stanniocalcin 1,STC-1)的表达情况及其与预后的关系。方法:Western印迹分析STC-1在宫颈癌细胞系的表达,免疫组织化学染色(immunochemistr y,IHC)检测STC-1在宫颈癌和正常组织的表达,分析STC-1表达水平与宫颈患者临床病理特征之间关系,通过网上数据库初步探索STC-1表达与预后的关系。结果:宫颈正常上皮细胞Ect1/E6E7细胞株中几乎检测不到STC-1,宫颈癌细胞株中STC-1呈不同程度表达;IHC结果显示:STC-1主要在宫颈细胞的胞质和胞膜中表达,与匹配的癌旁正常组织相比,STC-1蛋白水平在宫颈癌组织中更高(P<0.05),并且与患者淋巴结转移正相关(P=0.038);另外,通过对数据库中宫颈癌患者的样本分析发现:高表达STC-1的宫颈癌患者预后更差(P<0.01)。结论:STC-1表达与宫颈患者的总体存活负相关,STC-1可能具有预测宫颈癌患者预后生存时间的潜在价值。

Objective： To investigate the expression of stanniocalcin 1（ STC-1） in cervical cancer and its relation with prognosis. Methods： STC-1 protein expression in cervical cancer cell lines was determined by Western blot. We examined STC-1 expression in human cervical cancer tissues by immunohistochemical staining（IHC）, and investigated the clinical correlation between STC-1 tissue levels and the clinicopathological characteristics of cervical cancer patients. Kaplan-Meier curves for survival in cervical cancer patients were determined based on the analysis of STC-1 expression from online database. Results： STC-1 was markedly overexpression in all cervical cancer cell lines compared with normal cells. IHC results revealed that STC-1 was mainly expressed in the cell membrane and plasma. STC-1 protein levels were significantly higher in cervical cancer tissues when compared with matched non-cancerous tissues（P〈0.05）, and associated with lymph node metastasis（P=0.038）. Kaplan-Meier curves for survival analysis of online database showed that patients with high STC-1 expression had poor prognosis（P〈0.01）. Conclusion： STC-1 expression is correlated with lymph node metastasis of cervical cancer patient and associated with prognosis, may be a potential bio-marker for prognosis.