HMGA1在乳腺癌中的表达及临床意义

Expression of HMGA1 in breast cancer and its clinical significance

目的探讨高迁移率族蛋白A1(HMGA1)在乳腺癌组织中的表达及临床意义。方法应用实时定量荧光聚合酶链反应检测65例乳腺癌组织及配对的癌旁正常乳腺组织和40例乳腺纤维腺瘤组织中HMGA1 mRNA的表达情况;应用免疫组织化学法检测石蜡包埋的120例乳腺癌组织、46例乳腺纤维腺瘤组织和43例乳腺腺病组织中HMGA1的蛋白表达水平。结果乳腺癌组织中HMGA1 mRNA的表达显著高于癌旁正常乳腺组织及乳腺纤维腺瘤组织(P<0.01);乳腺癌组织中HMGA1蛋白的阳性表达率显著高于乳腺纤维腺瘤组织(P<0.01)和乳腺腺病组织(P<0.05),乳腺癌组织中HMGA1蛋白的表达与患者的年龄、淋巴结是否转移及TNM分期有关(P<0.01,P<0.05),三阴性乳腺癌HMGA1蛋白的阳性表达率明显更高(P<0.05)。结论HMGA1在乳腺癌组织中表达较癌旁正常组织和良性疾病组织明显升高,并与临床分期有关,有望成为乳腺癌诊治中的一个新的靶点。

Objective To investigate the expression and clinical significance of high mobility group A1 （HMGA1） in breast cancer. Methods The expression of HMGA1 mRNA was detected by real-time fluorescent quantitative polymerase chain reaction in 65 cases of breast cancer and the matched normal tissues,40 cases of fibroadenoma of breast tissues;immunohistochemical method was used to detect the expression of HMGA1 protein in 120 cases of breast cancer tissues, 46 cases of breast fibroadenoma and 43 cases of adenosis of breast tissues. Results The ex- pression of HMGA1 mRNA in breast cancer was significantly higher than the matched normal tissues and fibroade- noma of breast tissues（P 〈 0. 01 ）. The positive expression rate of HMGA1 protein in breast cancer was significantly higher than in fibroadenoma of breast（ P 〈 0. 01 ） and adenosis of breast tissues（ P 〈 0. 05 ）. The expression of HM- GA1 protein was associated with age, lymph node metastasis and TNM stage（P 〈0. 01 ,P 〈0. 05）. The positive expression rate of HMGA1 protein in triple negative breast cancer was significantly higher（ P 〈 0. 05 ）. Conclusion Compared with the normal tissues and benign disease group, HMGA1 expression in breast cancer tissue increased significantly. It is expected to become a new marker for diagnosis and treatment of breast cancer.