Micro-CT用于分析种植体周围骨小梁情况及相应ROI选择的初探

Analysis of trabecular bone around the implant by Micro-CT and the corresponding choice of ROI

初探微计算机断层扫描技术(Micro-CT)分析种植体周围骨小梁情况,介绍运用特定的感兴趣区域(ROI)选择办法,对比分析不同时期种植体-骨界面骨结合情况。实验动物为8只雄性Beagle犬,将每只犬双侧下颌第3、4前磨牙微创拔除,牙龈翻瓣后选择相应拔牙窝的合适位置即刻植入种植体(规格为长度10.0 mm,直径3.8 mm)各1颗。术后4周,随机处死4只实验犬,其余4只于种植术后8周时处死,术后4周及8周共有32枚种植体样本,运用Micro CT扫描,使用特定ROI选择方法进行分析研究对比。结果显示术后4周与8周种植体周围骨密度及骨小梁数目、厚度、形态等方面差异均有统计学意义(P<0.05)。本实验运用的选择ROI方法客观、合理、有效,最终ROI分析结果符合传统骨愈合及种植体-骨结合理论,为临床种植学研究提供了指导。

Microcomputed tomography（Micro-CT） was used to analyze the situation of trabecular bone around theimplant, and the specific region of interest（ROI） selection method was used to analyze the osseointegration of im- plant-bone interface in different periods. Eight male Beagle dogs were used in this experiment. The bilateral man- dibular third and fourth premolars were removed by minimally invasive operation, After the gingival flap. The im- plant （ 10. 0 mm in length and 3.8 mm in diameter） was implanted in appropriate location immediately. Four ex- perimental dogs were randomly assigned at 4 and 8 weeks postoperatively. A total of 32 implant samples were ob- tained. Micro CT scans were performed using the specific ROI selection method of this experiment, and the final re- suits were compared. Compared 4 weeks with 8 weeks, BV/TV, IS, Tb. Th, Th. N, BS/BV, Th. Pf, SMI, Po were all displayed significantly difference（P 〈 0.05 ）. The ROI method in this experiment was objective, reasonable and ef- fective. The results of the final ROI analysis consistent with the traditional bone healing and implant-bone-binding theory, which provided guidance for clinical implant research.