急性高原低氧应激损伤大鼠CRH水平研究

CRH biomarker on rats under acute plateau hypoxia stress

目的:探讨急性低氧应激条件下大鼠促肾上腺皮质激素释放激素(corticotrophin releasing hormone,CRH)水平的变化以及急性低氧对大鼠心、脑、肾的损伤程度。方法:(1)在低压氧舱中模拟高海拔低氧,测定大鼠血浆CRH含量;(2)用光镜下观察组织切片法及免疫荧光标记法观察大鼠心、脑、肾组织细胞损伤程度。结果:模拟高海拔低氧暴露24h后,各种组织标本显示随着海拔高度升高大鼠心、脑、肾的损伤程度加重。海拔5 500m组相对对照组CRH含量均值为9.10pg/ml(P<0.01);海拔7 500m组相对对照组血浆CRH含量均值为22.46pg/ml(P<0.01);7 500m组相对5 500m组血浆CRH含量均值为13.35pg/ml(P<0.01)。结论:急性高原低氧应激条件下CRH分泌水平随着海拔高度升高而增高,并且这种变化随低氧程度的加重而增强,且大鼠重要脏器损伤程度随着海拔高度的升高而加重。

Objective：To explore the changes of the levels of corticotrophin releasing hormone（CRH）in rats under acute hypoxic stress and the degree of injury of heart,brain and kidney in rats with acute hypoxia.Methods：（1）The plasma CRH content of rats was measured in lowaltitude oxygen tank.（2）The injury degree of rat heart,brain and kidney were observed under light microscope and by immunofluorescence labeling.Results：After simulating hypoxic exposure to 24 h,all kinds of tissue samples showed that the injury degree of heart,brain and kidney increased with altitude.Mean content of CRH in 5 500 m group vs control group was 9.10pg/ml（P0.01）,the altitude of 7 500 mgroup vs control group was 22.46pg/ml（P0.01）,7 500 mgroup vs 5 500 mgroup was 13.35pg/ml（P0.01）.Conclusion：The CRH level is significantly increased in the acute altitude hypoxia condition,and the changes are enhanced with the increase of the degree of hypoxia.