摘要
The anther cultures of Brassica hirta underwent pollenembryogenesis and callusing,which showed a wide range of chromosome numbers varying from 9 (n=12) to a highly polyploid.For embryogenesis,pretreatment of floral buds in 0.4 M sucrose solution for 72 hrs at 4℃ was superior to freshly cultured anthers.Culture temperature of 30℃ for 14 days before maintenance of cultures at 25℃ was significantly beneficial for embryo yield in comparison to cultures continuously incubated at 25℃.Dark treatment during culture was more effective for pollen-embryo yield.
The anther cultures of Brassica hirta underwent pollen-embryogenesis and callusing, which showed a wide range of chromosome numbers varying from 9 (n=12) to a highly polyploid. For embryogenesis, pretreatment of floral buds in 0.4M sucrose solution for 72 hrs at 4℃ was superior to freshly cultured anthers. Culture temperature of 30℃ for 14 days before maintenance of cultures at 25℃ was significantly beneficial for embryo yield in comparison to cultures continuously incubated at 25℃. Dark treatment during culture was more effective for pollen-embryo yield.
关键词
油料作物
白芥
花粉-胚胎发生
染色体变异性
Pollen-embryogenesis, anther culture, Brassia hirta,chromosomal variabiliiy.
