基于三维响应曲面模型分析当归-红花补血功效的配伍规律

Compatibility Law Analysis of Tonifying Blood of Couplet Medicines of Angelicae Sinensis Radix and Carthami Flos by Three-dimensional Response Surface Model

目的:应用多指标综合指数法与三维响应曲面分析法研究当归-红花(GH)补血效应的配伍规律。方法:采用注射乙酰苯肼+环磷酰胺诱导的小鼠血虚模型,将实验动物分为正常组、模型组、当归-红花不同配比(1∶0,4∶1,2∶1,3∶2,1∶1,2∶3,1∶2,1∶4,0∶1)组,按剂量0.01 m L·g^(-1)灌胃给药,连续7 d,观察小鼠血常规和脏器指数(胸腺、脾脏)的变化,建立当归和红花配伍后的三维响应曲面模型。结果:单用当归剂量为7.8 g·kg^(-1)·d^(-1)时,补血效果最好,总效应值5.50;单用红花剂量为0.52 g·kg^(-1)·d^(-1)时,补血效果最好,总效应值4.57。当归和红花配伍后,当归剂量在0.5~1.2 g·kg^(-1)·d^(-1)和红花剂量在0~0.5 g·kg^(-1)·d^(-1)时,表现为协同作用,在大部分剂量配比范围内(约50%)表现为相加作用,其次为拮抗作用。结论:当归和红花配伍后补血效应的协同作用范围较窄,强度也不是很明显,并在一定量比时还有拮抗作用。

Objective： To demonstrate the pharmacodynamic interaction in tonifying blood of couplet medicines of Angelicae Sinensis Radix and Carthami Flos by comprehensive index method and three-dimensional response surface method. Method： Based on mouse blood deficiency model induced by injection of acetylphenylhydrazine （APH） and cyclophosphamide （CTX）, the effect of this couplet medicines with different proportions （1：0, 4：1, 2：1, 3：2, 1：1, 2：3, 1：2, 14, 0：1） and different concentrations on the peripheral blood indexes and organ indexes were observed. Then all indexes were integrated and analyzed by relative equation. The three-dimensional response surfaces were gained by Matlab R2010a software. Result： When theywere used singly, the best doses of Angelicae Sinensis Radix and Carthami Flos were 7.80, 0.52 g·kg-1·d-1, respectively. Most dose （about 50% ） of this couplet medicines exhibited addition action, followed by antagonism, and little dose （0.5-1.2 g. kg - 1·d -1of Angelicae Sinensis Radix and 0-0.5 g· kg - 1. d - 1 of Carthami Flos） had synergetic effect. Conclusion ： The range of synergetic effect of this couplet medicines on tonifying blood is little, and the intensity is not obvious, but it has antagonism in a certain amout.