20(R)-人参皂苷Rg3抗角膜瘢痕机制研究

The Study of the 20(R) Ginsenoside Rg3 on Inhibition of Corneal Scar

目的:研究不同浓度的20(R)-人参皂苷Rg3(GRg3)对兔角膜成纤维细胞增殖和转化生长因子-β1(TGF-β1)表达的影响,探讨GRg3抑制角膜瘢痕增生的机制。方法:直接选用大白兔获取兔角膜成纤维细胞后进行原代培养,采用甲基噻唑基四唑(MTT)比色法观察不同浓度(10 mg·L^(-1),20 mg·L^(-1),50 mg·L^(-1),100 mg·L^(-1),150 mg·L^(-1),200 mg·L^(-1))GRg3对兔角膜成纤维细胞增殖作用的影响。应用反转录-PCR(RT-PCR)技术研究不同浓度GRg3对培养的兔角膜成纤维细胞TGF-β1m RNA表达的影响。结果:MTT法检测结果:与对照组比较,当GRg3浓度达到20 mg·L^(-1)及以上时,兔角膜成纤维细胞增殖均显著受抑制。当GRg 3浓度达到50 mg·L^(-1)及以上时,GRg3对兔角膜成纤维细胞TGF-β1 mRNA表达具有抑制作用。结论:GRg3具有抑制体外培养的兔角膜成纤维细胞增殖的作用,并随着GRg3浓度的增加,其抑制作用增强。随培养时间的延长,GRg3抑制作用增强。GRg3对兔角膜成纤维细胞TGF-β1mRNA表达具有抑制作用。

Objective To explore the effects of the different concentrations Ginsenoside Rg3 on cultured corneal fibroblasts of rabbit proliferation and the expression of TGF-[31mRNA so as to investigate the mechanism of the inhibition of corneal scar. Methods We observed the effects of the different concentrations （ 10 mg · L^-1 20 mg· L^-1 ,50 mg· L^-1,100 mg·L^-1,150 mg ·L^-1, 200 mg · L^-1） GRg3 on proliferation by tetrazotium （MTT） colorimetric assay and detected the expression of TGF-[31mRNA by RT-PCR on cultured corneal fibroblasts of rabbit. Results The OD values gained by MTT were significant different among the experiment groups and control ones. When the concentrations of GRg3 is above 20 rag/L, GRg3 inhibited fibroblastic proliferation on cultured corneal fibroblasts. When the concentrations of GRg3 is abo,ce 50 mg/L, GRg3 inhibited the expression of TGF-β 1 mRNA on cultured corneal fibroblasts. Conclusion GRg3 significantly inhibited fibroblastic proliferation and the expression of TGF-β1 mRNA on cultured corneal fibroblasts of rabbit in vitro. The effect of inhibition increased with the increasing concentrations of GRg3 and cultured time.