不同方法处理的九香虫对人胃癌SGC-7901细胞体外作用比较及其抑癌组分分布

Effect of Aspongopus chinensis Dallas by different treatments on human gastric cancer SGC-7901 cells in vitro and its antitumor component distribution

本实验探讨冷冻和传统中药炮制方法处理的九香虫对胃癌细胞增殖的影响及九香虫抑癌活性组分的体内分布。体外培养人胃癌SGC-7901细胞,采用四甲基偶氮唑盐比色法(MTT法)观察不同方法处理的九香虫各组分水溶液对SGC-7901细胞的体外抑制作用。结果发现,炮制九香虫蛋白浓度为50和100mg·L^(-1)时作用48h对SGC-7901细胞生长具有抑制作用,抑制率分别为13.45%和14.68%,而浓度达到200和400mg·L^(-1)时对SGC-7901细胞生长具有促进作用,抑制率为-7.94%和-82.50%;冷冻处理下九香虫不同浓度对SGC-7901细胞生长具有显著的抑制作用,该处理组蛋白浓度为50、100、200和400mg·L-1时抑制率分别为0.49%,3.82%,4.42%,39.33%。选取九香虫整虫及分解后的各部位处理组最大作用浓度比较,增殖抑制率为血淋巴>腹部>整虫>头部。因此,冷冻处理的九香虫对胃癌细胞抑制率更高,且在该条件下九香虫的抑癌活性组分主要分布于血淋巴和腹部。

This experiment was aimed to investigate the proliferation effects of Aspongopus chinensis Dallas by different treatments on the gastric cancer cells and the distribution of tumor suppressor active components under cold treatment.Human gastric cancer SGC-7901 cell was cultured in vitro and the same concentration of protein was prepared.Methyl thiazolyl tetrazolium（MTT）assay was used to observe inhibitory effect on SGC-7901 cells.The results showed that when the protein concentrations of A.chinensis Dallas were 50 and 100mg·L^-1 from traditional Chinese medicine processing method,the inhibitory rates to SGC-7901 cells for 48 h were 13.45% and 14.68%.However,the growth of SGC-7901 cells was strongly promoted by high concentration at 200 and 400mg·L-1,with the inhibitory rates of-7.94%and-82.50%respectively.While SGC-7901 cells were significantly inhibited by different concentrations of A.chinensis Dallas under the frozen processing,the inhibitory rates were 0.49%,3.82%,4.42% and 39.33% at the protein concentrations of 50,100,200 and 400mg·L^-1 respectively.Compared the maximum concentration of the whole body with its different dissected parts of A.chinensis Dallas,the inhibitory effects of the hemolymph and abdominal aqueous solution were higher than that of the head and whole body.Therefore,the cold treated A.chinensis Dallas had higher inhibitory rates on gastric cancer cells,and the anticancer active components were mainly distributed in the hemolymph and abdomen.