摘要
目的观察芍药苷(PF)对脂多糖(LPS)诱导的THP-1细胞炎症因子和三磷酸腺苷结合盒转运体A1(ABCA1)表达的影响。方法用含PF(10-8、10-7、10-6、10-5、10-4mol/L)培养基预处理细胞0.5 h,再用含LPS(1mg/L)培养基共同培养细胞24 h。ELISA检测细胞培养液上清中白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、白细胞介素8(IL-8)和肿瘤坏死因子α(TNF-α)水平,Western blot检测细胞中ABCA1蛋白的表达。结果与对照组比较,LPS组细胞上清液中IL-1β、IL-6、IL-8和TNF-α的水平显著性升高(P<0.05),ABCA1蛋白表达显著性下调(P<0.05)。与LPS组比较,LPS+PF(10^(-6)、10^(-5)和10^(-4)mol/L)组上清液中IL-1β、IL-6、IL-8和TNF-α的水平显著性降低(均P<0.05),ABCA1蛋白表达显著性上调(P<0.05),呈现浓度依赖性。结论芍药苷抑制LPS诱导的THP-1细胞炎症因子分泌和ABCA1表达下调。
Aim To investigate the effect of paeoniflorin (PF) on secretion of inflammatory factors and expres- sion of ATP-binding cassette transporter A1 (ABCA1) in THP-1 ceils. Methods After pretreatment with PF ( 10^-8, 10^-7, 10^-6, 10^-5 and 10^-4 mol/L) for 0.5 h, THP-1 cells was co-treated by LPS ( 1 mg/L) and PF for 24 h. Levels of interleukin- 1β ( IL- 1β), interleukin-6 ( IL-6), interleukin-8 (IL-8) and tumor necrosis factor-or (TNF-ot) in supernatant of cell culture medium were tested by enzyme linked immunosorbent assay (ELISA). Expression of ABCA1 in THP-1 cells were measured by Western blot. Results Compared with control group, the levels of IL-1α, IL-6, IL-8 and TNF-α in supernatant of cell culture medium were significantly increased (all P〈0.05) and expression of ABCA1 was sig- nificantly down-regulated in LPS group (P〈0.05). Compared with LPS group, the levels of IL-1β, IL-6, IL-8 and TNF-α in supernatant of cell culture medium were significantly decreased (all P〈0.05) and expression of ABCA1 was signifi- cantly up-regulated in LPS+PF ( 10^-6, 10^-5 and 10^-4 mol/L) group (all P〈0.05). Conclusion Paeoniflorin inhibits the secretion of inflammatory factors and down-reguhion of ABCA1 expression induced by LPS in THP-1 ceils.
作者
袁娜
朱明燕
杨宏发
曾高峰
王波
YUAN Na ZHU Ming-Yan YANG Hong-Fa ZENG Gao-Feng WANG Bo(Department of Emergency Department of Cardiovascular, the Second Affiliated Hospital of University of South China, Hengyang , Hunan 421001, China)
出处
《中国动脉硬化杂志》
CAS
北大核心
2017年第9期895-898,共4页
Chinese Journal of Arteriosclerosis
基金
衡阳市科技计划项目(2015KJ58)
关键词
芍药苷
脂多糖
炎症因子
三磷酸腺苷结合盒转运体A1
Paeoniflorin
Lipopolysaccharide
Inflammatory factors
ATP-binding cassette transporter A1