摘要
目的通过超快速液相色谱-三重四极杆飞行时间串联质谱(UFLC-Triple TOF-MS/MS)技术对厚朴主要化学成分进行分析鉴定。方法采用Agilent ZORBAX SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),以0.1%甲酸水溶液-乙腈为流动相梯度洗脱;质谱使用电喷雾(ESI)离子源,在正离子与负离子两种模式下采集数据。依据高分辨质谱提供的准分子离子峰和碎片离子的精确分子质量信息,结合标准品对照与参考相关文献数据,对厚朴进行成分鉴定。结果初步鉴定出24个化学成分,其中包括12个木脂素类、9个生物碱类和3个苯丙素苷类化合物。结论为进一步探究厚朴的药效物质基础和质量调控机制提供基础资料。
OBJECTIVE To qualitatively analyze the major chemical constituents of Magnoliae Officinalis Cortex by UFLC-Triple TOF-MS/MS.METHODS The analysis was carried out on an Agilent ZORBAX SB-C18 column（4.6 mm×250 mm,5 μm） with gradient elution of 0.1% formic acid-acetonitrile under both positive and negative ionization modes of ESI,respectively.The chemical components of Magnoliae Officinalis Cortex were identified by analyzing the accurate mass of molecular and product ions provided by UFLC-Triple TOF-MS/MS,comparison with reference standards and the literature data.RESULTS A total of 24 compounds were identified or tentative presumed in Magnolia Officinalis Cortex,including 12 lignans,9 alkaloids,and 3 phenylpropanoid glycosides.CONCLUSION This study provides the foundation and support for study on material foundation of efficacy and the overall assessment of quality of Magnoliae Officinalis Cortex.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2017年第20期1824-1831,共8页
Chinese Pharmaceutical Journal
基金
江苏高校优势学科建设工程资助项目(YSXK-2014)
江苏高校品牌专业建设工程资助项目(PPZY2015A070)
