CACNA1C基因多态性与精神分裂症的关联研究

An association study of CACNAIC gene polymorphism with schizophrenia

目的探讨L型钙离子通道cdC亚基（calcium channel，voltage-dependent，Ltype，Mpha 1C subunit，CACNA1C）基因多态性与精神分裂症的关联。方法纳入1432例精神分裂症患者及1153名正常对照，采用连接酶检测一聚合酶链反应（1igasedetectionreaction—polymerasechainreaction，LDR．PCR）方法对CACNAlC基因rs1006737、rs1024582位点进行基因分型，观察其等位基因及基因型与精神分裂症发病风险的关系。结果患者组rs1006737位点的基因分布（A、G等位基因频率分别为5．3％、94．7％，AA、AG、GG基因型频率分别为O．2％、10．2％、89．6％）与对照组（A、G等位基因频率分别为4．0％、96．0％，AA、AG、GG基因型频率分别为0．4％、7．4％、92．3％）比较，差异有统计学意义（P〈0．05），患者组rsl024582位点的基因分布（A、G等位基因频率分别为4．9％、95．1％，AA、AG、GG基因型频率分别为0．2％、9．3％、90．5％）与对照组（A、G等位基因频率分别为4．0％、96．O％，AA、AG、GG基因型频率分别为0．1％、7．8％、92．1％）比较，差异无统计学意义（P〉0．05）。rs1006737位点G等位基因（OR=1．332，95％CI=1．016～1．746）与精神分裂症相关联（P〈0．05）。结论本研究支持在汉族人群中CACNA1C基因rs1006737位点多态性与精神分裂症相关联。

Objective To explore the relationship of calcium channel,vohage-dependent,L type,alpha 1C subunit （CACNA1C） gene polymorphism with schizophrenia. Methods A total of 1 432 patients with schizophrenia and 1 153 healthy controls were selected using Ligase Detection Reaction-Polymerase Chain Reaction（LDR-PCR） to analyze the genotypes of CACNA1C gene （rs1006737 and rs1024582）.Results The allelic and genotypic distributions in rs1006737 between patients with schizophrenia （A, G allele：5.3%,94.7% ; A/A, A/G, G/G genotypes ： 0.2%, 10.2%, 89.6% ） and healthy controls （ A, G allele： 4.0%,96.0% ; A/A, A/G, G/G genotypes ： 0.4%, 7.4%, 92.3% ） had statistically significance after analysis （P〈0.05） .The allelic and genotypic distributions in rs1024582 between patients with schizophrenia （A, G allde ：4.9%, 95.1% ; A/A, A/G, G/G genotypes ： 0.2%, 9.3%, 90.5% ） and healthy controls （ A, G allele ： 4.0%,96.0% ; A/A, A/G, G/G genotypes ：0.1%,7.8%, 92.1% ） did not have statistically significance after analysis （P〉0.05）.The G allele of rs1006737 （OR= 1.332,95%CI= 1.016-1.746） was associated with the risk of schizophrenia （P〈 0.05 ）. Conclusion This research supports that CACNA1C gene rs 1006737 polymorphism is associated with schizophrenia in Han Chinese.