竹叶总黄酮对脂肪酸合酶及人乳腺癌细胞的抑制作用

Inhibitory Effects of Bamboo Leaves Flavonoids on Fatty Acid Synthase and Human Breast Cancer Cells

研究了毛竹、苦竹、阔叶箬竹、淡竹竹叶总黄酮对脂肪酸合酶(FAS)和人乳腺癌细胞(MDA-MB-231)的体外抑制活性。通过紫外分光光度法测定竹叶总黄酮对FAS的抑制作用,CCK-8法检测MDA-MB-231细胞的体外增殖抑制作用,流式细胞仪检测MDA-MB-231细胞凋亡作用。结果表明:毛竹、苦竹、阔叶箬竹、淡竹竹叶总黄酮粗提物对FAS抑制活性显著,其IC50依次为:112.43、153.29、161.32和195.09 mg/L;4种竹叶总黄酮粗提物对MDA-MB-231细胞具有显著体外抑制活性,且具有良好的量效关系。竹叶总黄酮的主要活性部位为大孔树脂70%和95%乙醇相;苦竹竹叶95%乙醇相对MDA-MB-231细胞抑制活性最强,当质量浓度100、200、400 mg/L时对MDA-MB-231细胞增殖抑制率分别为25%、44%和70%;凋亡率分别为11.1%、23.1%和38.7%。

The inhibitory effects of bamboo leaves flavonoids（ BLF） from the species of Pleioblastus amarus（ Keng） keng,Indocalamus latifolius（ Keng） Mc Clure,Phyllostachys heterocycla（ Carr.） Mitford Pubescens and P. glauca Mc Clure on the fatty acid synthase（ FAS） and human breast cancer cell MDA-MB-231 in vitro were studied. The inhibitory effects of bamboo leaves flavonoids on FAS were determined by UV spectrophotometry,and the inhibitory effects of MDA-MB-231 were detected by CCK-8 assay. Flow cytometry was used to detect the apoptosis of MDA-MB-231. The results showed that all four bamboo leaves flavonoids had significant inhibitory effects on FAS and MDA-MB-231 cells. The IC50 values of four flavonoids against FAS were112. 43,153. 29,161. 32 and 195. 09 mg/L,respectively. The inhibition effects of flavonoids on MDA-MB-231 followed the dose-effect relationship. Further studies showed that the 70% and 95% ethanol fraction of bamboo leaves flavonoids exhibited the strongest cytotoxicity. The inhibition rates against MDA-MB-231 at the mass concentration of 100,200,400 mg/L of 95% ethanol fraction of P. amarus were 25%,44% and 70%,and the apoptosis rates were 11. 1%,23. 1% and 38. 7%,respectively.