宁夏地区艾滋病患者感染HIV病毒pol区基因变异性研究

Study on the genetic variation of Pol region of HIV virus in AIDS patients in Ningxia

目的通过巢式聚合酶链式反应检测宁夏地区艾滋病患者感染HIV-1病毒的pol基因片段分析其变异情况,为艾滋病防治提供参考依据。方法采集艾滋病患者全血标本,提取病毒RNA,针对HIV-1病毒pol区设计引物,进行巢式PCR扩增,凝胶电泳鉴定后送测序。结果巢式PCR检测47份,成功扩增35分样本和获得pol区序列,长1 060 bp。宁夏地区HIV-1感染者中存在四种基因亚型,即CRF07-BC占65.7%、CRF08-BC占17.1%、CRF01-AE占11.4%、B亚型占5.7%,基因离散率为B亚型最大。结论 HIV-1基因型别趋于多样化,CRF07-BC重组亚型为主要流行毒株,B亚型在宁夏地区流行时间最长;吸毒人群和性活跃人群仍是艾滋病防治的重点人群。

Objective To study the characteristics of human immunodeficiency virus （H1V） gene sequence, sub -type distri- bution and origin of HIV strains, epidemic status and trends in Ningxia, to provide scientific evidence for AIDS prevention and control measures. Methods Antieoagulating blood samples were collected from HIV infection individuals and epidemiological data were ana- lyzed. Pol gene were amplified by nested PCR from the RNA extraction and then sequenced. The acquired sequences were compared with the international subtype references. The phylogenetic trees were constructed to determine the subtype of the samples. Results 47 samples were detected, 35 samples were PCR positive and the sequences were acquired. 23 cases were CRF07_BC （65.7%）, 6 cases were CRF08_BC （ 17.1% ）, 4cases were CRF01_AE （ 11.4% ） and 2 cases were subtype B （3.0%）, and the B subtype were the lar- gest genetic -distance. Conclusion The subtypes are tending to diversity, CRF07_BC is the dominant strain and shows a high preva- lence, drug users and sex active people still are the key of the HIV/AIDS prevention and control of the crowd.