儿童急性淋巴细胞白血病SOCS3 mRNA的表达水平与临床特点、早期治疗反应的相关性

Correlation of SOCS3 mRNA Expression Level with Clinical Characteristics and Early Response to Treatment in Pediatric Acute Lymphoblastic Leukemia

目的:观察细胞因子信号转导抑制因子-3(SOCS3 mRNA)在儿童急性淋巴细胞白血病(ALL)中的表达水平,分析SOCS3 mRNA表达水平高低与儿童ALL疾病状态、危险度之间的关系,探讨SOCS3 mRNA在儿童ALL疾病状态和危险度评估、靶向治疗中的应用。方法:采用SYBR Green荧光定量PCR的方法检测45例新诊断ALL患儿初诊时、诱导缓解期和13例正常儿童骨髓单个核细胞中SOCS3 mRNA表达水平;分别采用方差分析和秩和检验的方法分析SOCS3 mRNA表达水平与ALL危险度、临床特点的关系。同时采用免疫荧光组织化学染色联合激光共聚焦显微技术测定ALL患儿骨髓单核细胞中SOCS3 mRNA的位置与表达量。结果:45例患儿初诊时SOCS3mRNA表达水平明显低于正常儿童(P<0.05);诱导缓解期45例患儿的SOCS3 mRNA表达水平与正常儿童无显著差异(P>0.05);初诊时中、高危组患儿SOCS3 mRNA表达水平高于低危组(P<0.05);按照中位数法将45例患儿初诊时SOCS3 mRNA表达水平分为高表达组和低表达组,比较2组患儿临床特点发现,SOCS3 mRNA高表达组具有更高的外周血白细胞计数、乳酸脱氢酶水平和预后不良基因。此外,2组在危险度比较时SOCS3 mRNA高表达组危险度更高。结论:SOCS3 mRNA在初诊时下调而在疾病诱导缓解后恢复正常表达,SOCS3可以作为评价疾病状态的指标。SOCS3 mRNA的高表达使ALL危险度上调,SOCS3 mRNA可以作为评价ALL危险度的因子,通过调节SOCS3 mRNA表达水平治疗ALL或许可以成为治疗的新方向。

Obejective： To investigate the expression level of suppressor of cytokine signaling SOCS3 mRNA in children＇s acute lymphoblastic leukemia（ ALL）; to analyze the relationship betw een the expresion level of SOCS3 mRNA and disease status and risks of ALL,and to explore the application of SOCS3 mRNA in evaluation of ALL disease status,risk and target therapy. Methods： The expression levels of SOCS3 mRNA in bone marrow mononuclear cells from 45 cases of new ly diagnosed ALL at initial diganosis and induction remission and 13 normal children as controls w ere detected by SYBR Green fluorescence quantitative PCR; the correlation of SOCS3 mRNA expression level w ith risk and clinical characteristics of ALL w as analyzed by means of statistical method; the immunofluorescence histochemistry method and laser confocal microscopy w ere used to detect the sites and expression level of SOCS3 mRNA in bone marrow samples of ALL patients. Results： The SOCS3 mRNA expression level at initial diagnosis of 45 ALL patients w as significantly low er than that of normal controls（ P〈0.05）,and that in induction remission of 45 patients w as not significant different from normal controls（ P〈0.05）; the SOCS3 mRNA expression level at initial diagnosis of patients w ith standasd and high risk w as higher than that of patients w ith low risk（ P〈0.05）. The 45 patients w ere divided into high expression and low expression groups according to SOCS3 mRNA expression level at initial diagnosis by median method,comparison of clinical characteristics in 2 groups w as found that the SOCS3 mRNA high expression group had even more higher leukocyte count in peripheral blood,even more higher LDH level and much more poor prognostic genes; in addition,the high expression group show ed more higher risk in comparison betw een 2 group. Conclusion：The SOCS3 mRNA expression is dow n-regulated at initial diagnosis,w hile recovered to normal level after induction remission of disease,thus the SOCS3 can be used as an indicator for evaluation of disease status. The high expression of SOCS3 mRNA up-regulates the disease risk,therefore the SOCS3 mRNA can be used as a factor for evaluation of ALL risk. The treatment of ALL via regulation of SOCS3 mRNA expression level maybe can become a new w ay. The regulation of SOCS3 mRNA expression level maybe can become a new w ay for treatment of ALL.