NS-398联合硼替佐米对人多发性骨髓瘤RPMI 8226细胞的增殖抑制作用

Anti-proliferation Effect of NS-398 in Combination with Bortezomib on Multiple Myeloma RPMI 8226 Cells In Vitro

目的:观察NS-398能否增强多发性骨髓瘤RPMI 8226细胞对硼替佐米(BOR)的化疗敏感性。方法:NS-398与BOR联用后,用MTT法测定对体外培养的RPMI 8226细胞增殖的抑制作用;流式细胞术检测对细胞周期和凋亡的影响;ELISA法检测细胞的Caspase-3活性;Cox-2试剂盒定量检测各组细胞Cox-2的活性。结果:NS-398联合BOR对RPMI 8226细胞的增殖抑制率大于单用组(Q>0.85);NS-398联合BOR使RPMI 8226细胞阻滞于G0/G1期,细胞凋亡率明显高于单用组(Q>1.15);NS-398联合BOR组细胞的Caspase-3的活性高于单用组(Q>1.15)。结论:NS-398具有协同增强BOR体外抗骨髓瘤RPMI 8226细胞的作用。

Objective： To investigate if NS-398 could enhance the chemosensitivity of Bortezomib（BOR） on human multiple myeloma RPMI 8226 cells. Methods： After the treatment of NS-398 combined with BOR,MTT assay was used to detect the proliferation inhibition effect on human multiply myeloma RPMI 8226 cells in vitro,Flow cytometry was used to analyze their effect of apoptosis and cell cycle; the caspase-3 activity of different treatment group was detected by using ELISA and the activity of Cox-2 was measured by using Cox-2 activity assay kit. Results： The inhibitory rate of NS-398 combined with BOR was higher than that of NS-398 or BOR alone（Q 〉0. 85）. After treatment of NS-398 combined with BOR,the percentage of cells arrested in the G0/G1 phase and apoptotic rate were both higher than that of treatment with each drug alone（Q 〉1. 15）. The caspase-3 activity in cells treated with combined of NS-398 and BOR was significantly higher than that of treatment of each drug alone（Q 〉1. 15）. Conclusion： NS-398 combined with BOR shows a synergistic effect on the growth inhibition of RPMI 8226 cells in vitro.