FeCl_3损伤小鼠肠系膜小动脉血栓模型的主要影响因素

Main Factors Influencing FeCl_3-Induced Mouse Mesenteric Arteriole Thrombosis Model

目的:探讨FeCl_3损伤小鼠肠系膜小动脉血栓模型的主要影响因素。方法:通过下腔静脉获取C57Bl/6小鼠全血,用Calcein-AM标记洗涤血小板,将荧光标记的血小板输入受体鼠眼球后静脉窦,进入血液循环系统。实验分为回输血小板数1×10~7、1×10~8和2×10~8 3组;小鼠的周龄分为3-6、6-10和>10周3组;FeCl_3浓度分为6%、12%、24%、48%4个组。血管堵塞时间在10-20 min内,且栓子形成后15 s不脱落即为此模型建立成功。观察回输血小板的数量、小鼠的周龄、FeCl_3的浓度、滤纸片的大小等因素对建立肠系膜小动脉血栓模型的影响。结果:3-6周龄雄鼠血管堵塞时间为16 min,时间相比6-10周的(25 min)短(P<0.05),>10周(38 min)短(P<0.01);1×10~8和2×10~8的回输血小板数血管堵塞时间约15-18min,时间相比1×10~7(30 min)的短;6%和12%的FeCl_3的血管堵塞时间在15-20 min之间,但是24%和48%的FeCl_3一般在10 min以内就会出现血管堵塞现象。结论:FeCl_3损伤的小鼠肠系膜小动脉血栓模型的成功建立受很多因素影响。3-6周龄C57Bl/6雄鼠、回输血小板数(1-2)

Objective： To investigate the factors that influence FeCl3-induced mouse mesenteric arteriole thrombosis model. Methods： Platelets were isolated from donor mice and labeled with Calcein-AM. Mice were transfused intravenously with Calcein-AM labeled platelets. The influence of mouse ages（3-6 weeks,6-10 weeks and 10 weeks）,transfused platelets counts（1 × 10^7,1 × 10^8 and 2 × 10^8 platelets） and concentrations of FeCl3（6%,12%,24% and48%） on FeCl3-induced thrombosis model were compared. Results： The occlusion time was 16 min for mice aged 3-6 weeks,which was shorter than that for 6 mice aged 6-10 weeks（25 min）（P〈 0. 05） and that for mice aged 10 weeks（38 min）（P 0. 01）. The occlusion time resulting from transfusion of 1 × 10^8 and 2 × 10^8 of pletclets was 15-18 min,which was shorter than that of transfusion 1 × 10^7 platelets（30 mins）. The occlusion time resulting from transfusion of 6% and 12% FeCl3 was from 15 to 20 min,however the transfusion of 24% and 48% FeCl3 all in all leads to vessel occlusion within 10 min. Conclusion： The factors influencing the success of FeCl3-induced mouse thrombosis model are more. Transfusion of 1 × 10^8 to 2 × 10^8 labeled platelets to 3-6 week-old mice,and 6% to 12% of FeCl3 should be used to induce thrombosis,and the experimental conditions should be optimized for this animal model,therefore,it is easier for us to set up a mouse mesenteric arteriole thrombosis model.