3种微孢子虫在我国西南部分地区腹泻患者中的流行情况调查

Investigation of the prevalence of Enterocytozoon bieneusi,Encephalitozoon intestinalis and Encephalitozoon cuniculi among diarrheal patients in the southwest of China

目的调查毕氏肠道微孢子虫(Enterocytozoon bieneusi)、肠炎微孢子虫(Encephalitozoon intestinalis)和兔脑炎微孢子虫(Encephalitozoon cuniculi)在我国西南地区的流行情况。方法收集了124份西南地区腹泻患者的粪便,通过显微镜镜检、PCR检测等手段,进行微孢子虫的分离与鉴定。结果毕氏肠道微孢子虫和肠炎微孢子虫的检出率较高,分别为7.26%(9/124)和3.23%(4/124),兔脑炎微孢子虫的检出率为0。结论西南地区腹泻患者有感染微孢子虫的情况,感染类型多为毕氏肠道微孢子虫和肠炎微孢子虫。

Objective To investigate the prevalence of Enterocytozoon bieneusi,Encephalitozoon intestinalis and Encepha- litozoon cuniculi among diarrheal patients in the southwest of China.Methods A total of 124 fecal specimens from patients with diarrhea syndrome were collected.The specimens were stained by modified trichrome （weber） and were examined under microscopy.The extracted DNA samples were evaluated by PCR amplification.Results The prevalence of E.bieneusi was 7. 26% （9/124）,and that of E.intestinalis was 3.23% （4/124） ,with E.cuniculi all negative.Conclusion The infection of mi- crosporidia did exist in the southwest of China,and the most common types in this area were E.bieneusi and E.intestinalis.