栀子苷对糖尿病模型大鼠认知功能障碍的改善作用研究

Study on the Improvement Effect of Geniposide on Cognitive Dysfunction in Model Rats with Diabetes

目的:研究栀子苷对糖尿病模型大鼠认知功能障碍的改善作用。方法:将50只大鼠随机分为空白对照组(蒸馏水)、模型组(蒸馏水)和栀子苷低、中、高剂量组(5、10、20 g/kg),每组10只。除空白对照组外,其余4组大鼠均饲以高糖高脂饲料+注射链脲佐菌素复制糖尿病模型。成模后,各组大鼠ig相应药物,每日1次,连续8周。末次给药后检测各组大鼠空腹血糖(FPG)、糖化血红蛋白(HbA_1c)水平,并检测Morris水迷宫实验的逃避潜伏期和Y迷宫实验的行为正确率。结果:与空白对照组比较,模型组大鼠FPG、HbA_1c水平明显升高,逃避潜伏期明显延长,Y迷宫行为正确率明显降低,差异均有统计学意义(P<0.05)。与模型组比较,栀子苷各剂量组大鼠上述指标均明显改善(P<0.05),且与剂量呈正相关(P<0.05)。结论:栀子苷可有效降低糖尿病模型大鼠血糖水平,改善其认知功能障碍。

OBJECTIVE：To study the improvement effect of geniposide on cognitive dysfunction in model rats with diabetes.METHODS：50 rats were randomly divided into blank control group（distilled water）,model group（distilled water）and geniposide low-dose,medium-dose,high-dose groups（5,10,20 g/kg）,10 in each group. Except for blank control group,rats in other 4 groups were fed with high-sugar and high-fat diet and injected streptozotocin to induce diabetes model. After modeling,rats ineach group intragastrically administrated relevant medicines,once a day,for 8 weeks. After last administration,fasting blood glucose（FPG）,glycosylated hemoglobin（HbA1c）levels,escape latency period in Morris water maze test,and correct rate of behaviors in Y maze test of rats in each group were detected. RESULTS：Compared with blank control group,FPG,HbA1c levels of rats in model group were obviously increased,escape latency period was obviously prolonged,correct rate of behaviors in Y maze test was obviously decreased,with statistical significances（P〈0.05）. Compared with model group,above-mentioned indexes in geniposide groups were obviously improved（P〈0.05）,which were positively correlated with dose（P〈0.05）. CONCLUSIONS：Geniposide can effectively reduce blood glucose level of model rats with diabetes and improve their cognitive dysfunction.