犬新孢子虫免疫相关蛋白筛选及其生物信息学分析

Screening on Immune Associated Protein of Neospora caninum and Its Analysis of Biological Information

利用新孢子虫感染小鼠血清对新孢子虫c DNA表达文库进行SEREX筛选,获得的阳性克隆经测序后进行Blast分析,并利用生物学软件对其编码的蛋白结构进行预测。结果显示,经筛选共获得了4个阳性克隆序列,分别为新孢子虫表面抗原SAG1,两个假定蛋白(XM_003882171.1)和(XM_003882600.1),以及一个未命名蛋白(XM_003885351.1)。研究共获得了4个新孢子虫免疫相关蛋白,为新孢子虫诊断及其疫苗研究等研究奠定了基础。

By SEREX, the cDNA expression library of Neospora caninum were screened using mice serum infected with Neospora caninum. The positive clones were sequenced and analyzed by the biology software. The results showed that four positive clones se- quence were obtained which were one surface antigen SAG1 of N.caninum, two putative protein （XM 003882171.1） and （XM_003882600.1）, and an unnamed protein（XM_O03885351.1） respectively. Four immune associated proteins were successfully ob- tained, which lay the foundation for the diagnosis and vaccine of Neospora caninum.