摘要
以姜油树脂的粗分离物为原料,采用减压梯度柱层析技术对粗分离物中的6-姜酚进行分离纯化,研究了洗脱剂种类、填料比、洗脱剂浓度、硅胶颗粒目数等因素对6-姜酚含量及收率的影响。结果表明,以300~400目硅胶为填料,填料比为100,石油醚、正己烷及乙酸乙酯为洗脱剂,洗脱剂密度增量为0.005g/m L,起始浓度范围为0.695~0.725 g/m L,在此工艺参数下对6-姜酚进行减压梯度柱层析分离纯化,可将其含量从55.03%提高到93.04%,收率大于83%。
With ginger oleoresin of crude extracts as raw material,the crude extracts of 6-gingerol were separated and purified by decompression gradient column chromatography. The effects of types of eluent,ratio of filling,elution of gradient,filler particle mesh factors on the content and yield of 6-gingerol were studied. The results showed that 300-400 mesh silica gel was used as filler and the packing ratio was 100. Petroleum ether,hexane and ethyl acetate were used as eluents and the density increment of them was 0.005 g/m L and the initial concentration range was 0.695 ~0.725 g/m L. Under these process parameters,the content of 6-gingerol could be increased from 55.03% to 93.04% and the yield was higher than 83%.
出处
《湖北农业科学》
2017年第19期3717-3719,3735,共4页
Hubei Agricultural Sciences
基金
贵州省社会发展科技攻关项目(2014SY3080)
关键词
姜油树脂
柱层析
6-姜酚
ginger oleoresin
column chromatography
6-gingerol
