不同中药总黄酮含量测定方法的选择研究

Research on Determination Method of Flavonoids of Different Traditional Chinese Medicine

微波辅助提取十种中药中的总黄酮,以芦丁为标准品,用五种不同的测定方法探讨不同中草药中总黄酮的含量,研究黄酮结构和测定方法之间的关系.结果表明:直接测定法更适合甘草、银杏叶和陈皮;NaNO_2-Al(NO_3)_3法更适合于山楂和黄芪;差示分光光度法更适合于野菊花、山楂、葛根和连翘;AlCl_3法更适合于陈皮;荧光光度法除山楂外,其它中草药测定均佳.根据总黄酮含量的测定方法的原理探讨不同中药中黄酮类物质的结构.

In order to investigate the best assay method of total flavonoids and the relationship between flavonoids structure and assay method, total flavonoids of ten traditional Chinese medicines were extracted by microwave technology and determined by different methods. The results showed that the direct methods were suitable for testing the total flavonoids of Glycyrrhiza uralensis, leaves of Ginkgo biloba and pericarpium citri reticulatae, the method of NaNO2 - Al（ NO3 ） 3 was suitable for Crataegus pinnatifida and Astragalus raembranaceus , the method of differential speetrophotometry was suitable for Chrysanthemum indicum, Crataegus pinnatifida, Pueraria lobata, and Forsythia suspensa. The method of AlCl3 was suitable for pericarpium citri retieulatae, and the method of fluorospeetrophotometry was suitable for all apart from the hawthorn. The determination of total flavonoids in the Chinese medicines was affected by various factors, such as the types of flavonoids, the choice of measurement method, etc.