摘要
Our previous studies showed a predominance of high molecular weight protein group in tumor nuclear matrices. contrary to normal cells, Proteins of this group are Preferentially phosphorylated. Phosphoproteins of hepatoma nuclear matrix are selectively subjected to rapid proteolysis. By alkali treatment and a monoclonal antibody against phosphotyrosyl residue the presence of two high molecular weight bands of phosphotyrosyl-containing proteins was defected in nuclear matrices of tumor but not of normal liver cells. High molecular weight protein group of tumor nuclear matrices revealed also a rapid turnover and preferential incorporation of labeled amino acids selectively inhibited by chioramphenicol.
Our previous studies showed a predominance of high molecular weight protein group in tumor nuclear matrices. Contrary to normal cells, proteins of this group are preferentially phosphorylated. Phosphoproteins of hepatoma nuclear matrix are selectively subjected to rapid proteolysis. By alkali treatment and a monoclonal antibody against phosphotyrosyl residue the presence of two high molecular weight bands of phosphotyrosyl-containing proteins was detected in nuclear matrices of tumor but not of normal liver cells. High molecular weight protein group of tumor nuclear matrices revealed also a rapid turnover and preferential incorporation of labeled amino acids selectively inhibited by chloramphenicol.
关键词
肿瘤细胞
核基质
高分子量蛋白
生物合成
磷酸化
磷蛋白
Animals
Antigens, Nuclear
Hela Cells
Humans
Male
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Molecular Weight
Neoplasms
Nuclear Matrix
Nuclear Proteins
Phosphoproteins
Phosphorylation
Phosphotyrosine
Rats
Rats, Wistar
Research Support, Non-U.S. Gov't