迎春花试管培养技术的研究

In Vitro Cultivation Technology of Winter jasmine L.

旨在研究不同培养条件对迎春花不定芽再生和生根状况的影响,并筛选出最适培养条件。以迎春花带芽茎段为材料,通过诱导愈伤组织、愈伤组织分化新芽、芽生根等步骤建立迎春花离体快速繁殖体系。研究结果表明,适合迎春花愈伤组织诱导的培养基为WPM+3.0 mg/L6-BA+0.5 mg/LNAA,诱导率高达100%;适合迎春花芽伸长诱导的培养基为WPM+2.0 mg/L6-BA+0.5 mg/LNAA,诱导率达56.8%;适合迎春花生根诱导的培养基为1/2 WPM+0.5 mg/L NAA,生根率高达85%,根系发达,试管苗生长健壮。培养条件为光照强度1500 lx,光照时间12 h/d,温度25℃。植物生长调节剂种类及质量浓度对迎春花不定芽的出芽时间和再生率以及生根率有明显影响。

To study the effects of different culture conditions on regeneration of adventitious bud and rooting of Winter jasmine,and screen the optimum culture condition,we used flower stems with buds of Winter jasmine as materials.By means of inducing the callus,callus differentiation of shoots and rooting,the rapid propagation system of Winter jasmine was established.The results showed that the effective medium for callus-inducing was WPM＋3.0 mg/L6-BA＋0.5 mg/LNAA,and the ratio was 100% .The effective medium for stem-inducing was WPM＋2.0 mg/L 6-BA＋0.5 mg/L NAA,and the ratio was 56.8% .The effective medium for rooting was1/2 WPM＋0.5 mg/L NAA,and the rooting rate was 85% ,roots were flourishing,and the test-tube plantlet was strong.The culture condition was illumination intensity 1500 lx,illumination time 12 h/d and culture temperature 25℃.The type and concentration of growth regulator had obvious effects on sprouting time and regeneration rate of adventitious bud.