反相高效液相色谱法测定利卡西平血药浓度

The determination of licarbazepine concentration in patients' plasma by RP-HPLC

目的:建立利卡西平血药浓度测定方法。方法:采用反相高效液相色谱法,色谱柱:Waters sunfire C_(18)柱(4.6 mm×250 mm,5μm);流动相:甲醇-水-三氟乙酸(600:400:0.5);流速:1.0 mL·min^(-1);柱温:40℃;检测波长:230 nm;进样量:20μL。内标物:盐酸赛庚啶。结果:以利卡西平与内标物峰面积之比(y)对利卡西平血清浓度(C)进行线性回归,得回归方程:C=1.227×10~2 y–0.364(r^2=0.999 6,n=7),结果表明利卡西平在5.0~100.0μg·mL^(-1)范围内样品回收率高(平均回收率为99.80%,RSD=1.25%)。结论:本方法操作简便、精密度好,可用于利卡西平的治疗药物监测。

Objective：To set up the HPLC method for determination of licarbazepine concentration in patient＇s plasma.Methods：The residues were analyzed by RP-HPLC method.Waters sunfire C18 column（4.6 mm × 250 mm,5 μm） was used,methanol-water-trifluoroacetic acid（600 ：400 ：0.5） was used as mobile phase.The flow rate was 1.0 mL·min^-1.The detection wavelength was set at 230 nm and the column temperature was 40 ℃.The sample size was 20 μL.Cyproheptadine hydrochloride was used as internal standard.Results：Linear regression analysis was made by licarbazepine serum concentration（C） and the peak area ratio（y） of licarbazepine vs internal standard.The regression equation was C = 1.227 × 10^2 y-0.364（r^2 = 0.999 6,n = 7）.The results showed that the recovery ratio of licarbazepine was high in the range of 5.0-100.0 μg·mL^-1（average recovery rate = 99.80%,RSD = 1.25%）.Conclusion：The method provided a simple and precise way for monitoring plasma concentration level of licarbazepine,and this method can be used for therapeutic drug monitoring of licarbazepine.