急性高眼压作用下视神经轴浆运输与视网膜光学功能的关系

Relationship between the axonal transport of the optic nerve and the optical function of the retina in acute high intraocular pressure

目的:研究急性高眼压下视神经轴浆运输与视网膜光学功能之间的关系。方法:通过前房灌注的方法制造急性高眼压动物模型;利用罗兰电生理仪进行闪光视网膜电图和视觉诱发电位的测量。在玻璃体注射荧光染料后,利用激光共聚焦显微镜观察视神经轴浆运输,并利用苏木素-伊红染色,观察视乳头的形态变化。结果:当眼压为60 mm Hg并维持2 h,闪光视网膜电图的a波幅值明显降低,视网膜外层功能可能发生变化。当眼压为100 mm Hg并维持4 h,闪光视网膜电图的a波、b波和Ops的幅值以及闪光视觉诱发电位的幅值明显降低,视网膜内外层光学功能出现损伤,此时视神经的轴浆运输明显改变,视乳头形态也发生了变化。结论:急性高眼压下视神经轴浆运输与视网膜光学功能之间可能存在一定的关系。

Objective To study the relationship between the optical function of the retina and the axonal transport of the optic nerve in animal models with acute high intraocular pressure. Methods The animal models with acute high intraocular pressure were built by anterior chamber perfusion. The flash electroretinogram and visual evoked potential were measured using Roland visual electrophysiological instruments. After intravitreally injecting fluorescent dye, the axonal transport of the optic nerve was examined by confocal laser scanning microscope; the morphological change of the optic nerve head was observed by HE staining.Results When the intraocular pressure was kept at 60 mm Hg for 2 h, the amplitude of a-wave in the flash electroretinogram decreased obviously, which suggested the function of the outer layer of the retina could be damaged. When the intraocular pressure was kept at 100 mm Hg for 4 h, the amplitude of a-wave, b-wave and oscillatory potentials in the flash electroretinogram and the wave of flash visual evoked potential was dramatically decreased, which indicated the retinal dysfunction. At the same time, the axonal transport of the optic nerve changed significantly and the morphology of the optic nerve head also changed. Conclusion Some relationships might exist between the axonal transport of the optic nerve and the optical function of the retina in acute high intraocular pressure.