血清miR-146a变化与强直性脊柱炎的相关性

Correlation between serum miR-146a changes and ankylosing spondylitis

目的:探究强直性脊柱炎血清miR-146a表达情况,从而发现潜在的强直性脊柱炎的生物标记分子。方法:qRTPCR检测强直性脊柱炎患者和正常人血清miR-146a表达水平。根据脊柱后凸的程度将患者分为A(后凸畸形<70%)和B(后凸畸形≥70%)两组。疾病的活性以及患者功能状态分别由强直性脊椎炎疾病活动度指数(BASDAI)和强直性脊椎炎功能指数(BASFI)来表示。采用qRT-PCR检测A和B两组血清miR-146a表达水平,绘制受试者工作特征(ROC)曲线评价血清中miR-146a对强直性脊柱炎的诊断价值,同时将miR-146a表达量与临床指标进行相关性分析。结果:miR-146a在强直性脊柱炎血清组表达明显上调(P<0.05)。miR-146a的ROC曲线下面积值为0.917;且B组强直性脊柱炎患者血清中miR-146a表达水平显著高于A组(P<0.05)。此外,miR-146a表达水平同BASDAI之间存在显著相关性(r=0.5,P<0.05)。结论:血清中miR-146a表达检测可作为强直性脊柱炎诊断的辅助性生物标记。miR-146a表达水平也可能与疾病活性和胸腰椎后凸畸形的严重程度成一定的相关性。

Objective To discover biomarkers of potential ankylosing spondylitis by exploring the expression of serum miR-146a in patients with ankylosing spondylitis. Methods qRT-PCR was used to detect the expression of serum miR-146a in patients with ankylosing spondylitis and normal controls. According to the degree of kyphosis, the patients were divided into group A（kyphotic deformity70%） and group B（kyphosis deformity ≥ 70%）. The disease activity and the functional status of the patients were expressed by Bath ankylosing spondylitis disease activity index（BASDAI） and Bath ankylosing spondylitis functional index（BASFI）, respectively. The expression of miR-146a in group A and B was detected by qRT-PCR. The diagnostic value of serum miR-146a on ankylosing spondylitis was evaluated by receiver operating characteristic（ROC） curve. We also analyzed the correlation between the expression of miR-146a and clinical indicators. Results miR-146a was significantly up-regulated in the serum of patients with ankylosing spondylitis（P〈0.05）. The area under the ROC curve of miR-146a was 0.917. The expression level of miR-146a in serum of patients in group B was significantly higher than that of group A（P〈0.05）. In addition, the expression level of miR-146a was significantly correlated with BASDAI（r=0.5, P〈0.05）. Conclusion The expression of miR-146a in serum can be used as an auxiliary biomarker for the diagnosis of ankylosing spondylitis. The expression level of miR-146a may also be related to the disease activity and the severity of thoracolumbar kyphosis.