结膜松弛症中胶原蛋白的初步研究

Preliminary investigation on collagen inconjunctivochalasis

目的探讨Ⅰ、Ⅲ型胶原蛋白在结膜松弛症患者结膜组织中的表达情况及意义。方法基础实验研究。使用苏木精一伊红染色观察结膜组织中胶原蛋白的形态表现及采用免疫组化方法检测正常人结膜组织和结膜松弛症患者结膜组织中Ⅰ型胶原蛋白、Ⅲ型胶原蛋白的表达情况，并结合Image—Pro—Plus图像分析系统测定阳性染色结果的光密度值，半定量比较各组间Ⅰ、Ⅲ型胶原蛋白的含量变化。结果Ⅰ型胶原蛋白含量、Ⅲ型胶原蛋白含量与Ⅰ／Ⅲ型胶原蛋白含量在结膜松弛症组和正常组的结膜组织中的差异均有统计学意义（P〈0．05）；两组中Ⅰ型＋Ⅲ型胶原蛋白含量相比差异无统计学意义（P〉0．05）。结论结膜松弛症组中Ⅰ型胶原蛋白较正常组减少，而Ⅲ型胶原蛋白较正常组增多，故结膜松弛症组中Ⅰ／Ⅲ型胶原蛋白降低，从而导致结膜组织的机械稳定性降低，这可能是结膜松弛症发生的一个重要原因。

Objective To investigate the amount changes and significance of type Ⅰ and Ⅲ colla- gen in the conjunctiva tissue of the patients with conjunctivochalasis. Methods The morphologi- calmanifestation of collagen in conjunctival tissues were observed by hematoxylin-eosin staining. The expression of type Ⅰ and Ⅲ collagen by immunohistochemical technique in normal human conjuncti- va and patients with conjunctivochalasis, combined with the optical density valueof positive stainin- gresults was measured by Image-Pro-Plus image analysis system, the amount changes of semi quanti- tative comparison between the two groups of type Ⅰ and Ⅲ collagen. Results Theamounts of type Ⅰ collagen, type Ⅲ collagen and the type Ⅰ/Ⅲ collagen were significantly different in conjunctival tis- sues of conjunctivochalasis group and the normal group（P 〈0.05）;the type Ⅰ plus III collagen of the two groups had no statistical different（P 〉0.05）. Conclusions The type Ⅰ collagenin the conjunctivi- tis group is lower than in the normal group, while the type Ⅲ collagen is more than in the normal group, so that the type Ⅰ/Ⅲ collagen decrease in the conjunctivochalasis group, which result in the decrease of the mechanical stability of the conjunctival tissue and may be an important reason of conjunctivochalasis.