金花茶组培快繁体系的建立

Establishment of technique system of tissue culture on golden Camellia

建立一套完整的金花茶组培再生体系,包括无菌苗诱导、继代增殖、壮苗、生根、移栽等,且各阶段配方效果稳定.种子萌发诱导培养基:MS+6-BA 0.2 mg/L+NAA 0.2 mg/L+蔗糖3%的效果好,萌发率为82%;继代增殖培养基:改良MS+6-BA 1 mg/L+KT 1 mg/L+IBA 1.5 mg/L+蔗糖4%的有效苗最多,增殖系数为4.2;壮苗培养基以改良MS+6-BA 0.3 mg/L+IBA 1 mg/L+蔗糖3%的效果最好;生根培养:无菌苗基部浸泡500 mg/L IBA溶液2 min后接种于1/3改良MS+蔗糖1.5%培养基的生根率最高,达86%;移栽基质以V泥炭土∶V黄泥∶V河沙=2∶1∶1的混合基质较好,60 d后移栽成活率可达91%.

A technique system of tissue culture of golden camellia was established completely in the study, including asepsis seedling induced, subculture multiplication, growth-promoting, rooting and transplantation. The techniques were as follows：asepsis seedling induced culture media was MS＋6-BA0.2 mg/L＋NAA0.2 mg/L＋ Sucrose（3%）, induction rate was 82%;clump shots induction culture media was modified MS ＋6-BA 1 mg/L＋KT 1 mg/L＋IBA 1 mg/L＋ sucrose（4%）, multiplication coefficients reached 4.2; growth-promoting culture media was modified MS＋6-BA0.3 mg/L＋IBA1 mg/L ＋sucrose（3%）; rooting culture media was 1/3 modified MS ＋sucrose（1.5%）, sterile shoots dipped in 500 mg/L IBA solution for 2 min were inoculated on the rooting culture media, and their rooting？rate was up to 86%; transplantation media was peaty soil 50% ＋ yellow soil25%＋fine sand 25%, and transplanting survival rate could reach 91% after 60 days.