摘要
[目的]使用多种培养成分组合,筛选出一个适合山羊诱导性多能干细胞(induced pluripotent stem cells,i PSCs)的培养体系。[方法]采用慢病毒作为载体,携带多能因子,采用多种培养成分(培养基、血清和细胞因子)的组合培养山羊i PSCs,优化其培养体系。[结果]用FBS代替KSR可以提高克隆的形成效率以及克隆的质量,而用DMEM/F12代替KNOCKOUT-DMEM,i PSCs克隆的形成效率和克隆质量没有明显的改善。带有β-FGF的培养体系可以保持i PSCs未分化的状态,而培养体系中只加LIF不能维持i PSCs未分化的状态。[结论]该研究成功诱导出山羊i PSCs,且能在体外传代培养。
[ Objective ] A culture system suitable for goat-induced pluripotent stem cells (iPSCs) was screened by using combination of various culture components. [Method] With the lentivirus as vector carrying pluripotency factor,goat iPSCs were cultured with a variety of culture compo-nents (culture medium,serum and cytokine) to optimize the culture system. [Result]The forming efficiency and clone quality of clones could be improved by FBS instead of KSR. The forming efficiency and clone quality of iPSCs clones were not improved by DMEM /F12 instead of KNOCK- OUT-DMEM. The culture system with (3-FGF could maintain the undifferentiated state of iPSCs,while only LIF could only maintain the undifferen-tiated state of iPSCs in the culture system. [ Conclusion ] Goat iPSCs were successfully induced and cultured in vitro.
出处
《安徽农业科学》
CAS
2017年第30期131-133,168,共4页
Journal of Anhui Agricultural Sciences
基金
宁夏自然科学基金项目(NZ15071)
关键词
诱导性多能干细胞
重编程
培养体系
Induced pluripotent stem cells
Reprogramming
Culture system