miR-1202在宫颈癌组织中的表达及对HeLa细胞增殖及凋亡的影响

Expression of miR-1202 in cervical cancer tissues and its effect on proliferation and apoptosis of He La cells

目的探讨miR-1202在宫颈癌组织中的表达及其对宫颈癌He La细胞增殖及凋亡的影响。方法采用实时定量PCR检测29例宫颈癌组织及相对应的癌旁正常组织中miR-1202的表达;构建miR-1202过表达载体及合成miR-1202抑制剂,并转染宫颈癌He La细胞;He La细胞被随机分为4组:对照组(miR-control)、miR-1202过表达载体组(miR-1202)、miR-1202抑制剂阴性对照组(Inhibitor NC)、miR-1202抑制剂组(miR-1202 inhibitor);MTT法检测miR-1202对宫颈癌细胞增殖的影响;流式细胞术分析miR-1202对细胞周期和凋亡的影响。结果 miR-1202在宫颈癌组织中的表达显著下调(P<0.01);miR-1202过表达载体组与miR-control组相比,OD值显著降低(P<0.01),G1/G0期细胞数显著增加(84.63%±5.08%;P<0.01),S期(11.56%±2.51%)和G2/M期(4.62%±1.75%)细胞数显著减少(P<0.01),早期凋亡(19.68%±2.66%)和晚期凋亡(10.89%±1.94%)细胞数均显著增加(P<0.01);而miR-1202 inhibitor组与Inhibitor NC组相比,OD值显著升高(P<0.01),G1/G0期细胞数量显著减少(43.68%±4.86%;P<0.01),S期(38.94%±2.86%)和G2/M期(18.65%±1.82%)细胞数量显著增加(P<0.01),早期凋亡(2.06%±1.33%)和晚期凋亡(2.85%±1.22%)细胞数均显著减少(P<0.01)。结论 miR-1202在宫颈癌组织中下调,可抑制宫颈癌He La细胞的增殖,并诱导细胞凋亡。

Objective To investigate the expression of miR-1202 in human cervical cancer tissues and its effects on proliferation and apoptosis of He La cells. Methods The qRT-PCR was used to detect the expression of miR-1202 in 29 cases of cervical cancer samples and control tissues. MiR-1202 overexpression vector was constructed. MiR-1202 inhibitor was synthesized. MiR-1202 overexpression vector and miR-1202 inhibitor were transfected into He La cells. He La cells were divided into four groups： control group（ miR-control）,miR-1202 overexpression vector group（ miR-1202）,miR-1202 inhibitor negative control group（ inhibitor NC） and miR-1202 inhibitor group（ miR-1202 inhibitor）. MTT assay was used to analyze the effects of miR-1202 on proliferation of cervical cancer He La cells. The effects of miR-1202 on the cell cycle and apoptosis of cervical cancer He La cells were observed by flow cytometer. Results The expression of miR-1202 significantly down-regulated in cervical cancer tissues（ P 〈 0. 01）. Compared with miR-control,miR-1202 overexpression vector decreased the OD value（ P 〈 0. 01）,increased the proportion of G1/G0 phase cells（84. 63% ±5. 08%,P 〈0. 01）,reduced the proportion of S and G2/M phase cells（11. 56% ±2. 51% and 4. 62% ±1. 75%,P 〈 0. 01）,and increased the proportion of early apoptosis and late apoptosis（19. 68% ±2. 66% and 10. 89% ±1. 94%,P 〈 0. 01）. Compared with inhibitor NC,miR-1202 inhibitor increased the OD value（ P 〈 0. 01）,diminished the proportion of G1/G0 phase cells（43. 68% ±4. 86%,P 〈 0. 01）,increased the proportion of S and G2/M phase cells（38. 94% ±2. 86% and 18. 65% ±1. 82%,P 〈 0. 01）,and reduced the proportion of early apoptosis and late apoptosis（2. 06% ±1. 33% and 2. 85% ±1. 22%,P 〈 0. 01）. Conclusion The expression of miR-1202 decreases in cervical cancer tissues,and it can inhibit the proliferation of cervical cancer cells and induce the apoptosis.