Real-time PCR方法检测早产儿脐带血中CMV-DNA、HSV1-DNA和HSV2-DNA的表达

The expression of CMV-DNA,HSV1-DNA and HSV2-DNA in umbilical cord blood of premature infants by real-time PCR

目的探讨早产儿脐带血中巨细胞病毒(CMV)、单纯疱疹病毒Ⅰ(HSV1),单纯疱疹病毒Ⅱ(HSV2)的感染与早产的相关性。方法采用实时荧光定量PCR(real-time PCR),对我院2015年6月1日-2016年12月31日出生的89例早产儿(研究组)和235例正常足月儿(对照组)脐带血进行检测。结果 89例早产儿中CMV-DNA检出10例,阳性率为11.24%,HSV1-DNA检出5例,阳性率为5.62%,HSV2-DNA检出4例,阳性率为4.49%。235例足月儿中CMV-DNA检出4例,阳性率为1.70%,HSV1-DNA检出2例,阳性率为0.85%,HSV2-DNA检出0例,阳性率为零。早产儿的CMV、HSV1、HSV2的感染率均高于足月儿,差异比较有统计学意义(P<0.05)。结论脐带血中CMV、HSV1、HSV2感染与早产的发生有明显相关性;且通过real-PCR方法检测较传统的检测方法敏感性强,准确度高。

Objective：To investigate the relationship between the infection of CMV,HSV1 and HSV2 in premature infant′s umbilical cord blood and premature delivery. Methods：Umbilical cord blood of 89 premature infants and 235 healthy term infants from June 1,2015 to December 31,2016 were detected by real-time PCR. Results：In 89 premature infants,10 cases（11.24%）were CMV-DNA positive,5 cases（5.62%）were HSV1-DNA positive,4 cases（4.49%）were HSV2-DNA positive. In 235 healthy term infants,4 cases（1.70%）were CMV-DNA positive,2 cases（0.85%）were HSV1-DNA positive,and all cases were HSV2-DNA negative. The infection rates of CMV,HSV1,HSV2 in premature infants were higher than those in healthy term infants,and the differences were statistical significant（P0.05）. Conclusion：The infections of CMV,HSV1 and HSV2 can be early identified by real-time PCR. This method′s susceptibility and accuracy are stronger than traditional detection methods.