石榴皮鞣质对大鼠肝药酶活性的影响

Effect of Tannins from Pericarpium Granati on Hepatic Microsomal Enzyme in Rats

目的:研究石榴皮鞣质对正常大鼠肝药酶活性的影响。方法:将30只Wistar大鼠随机分为5组:空白对照组,石榴皮鞣质高、中、低剂量组,苯巴比妥钠阳性对照组。空白对照组给予生理盐水,石榴皮鞣质高、中、低剂量组分别按150,100,75mg·kg^(-1)·d^(-1)连续灌胃给药7 d,苯巴比妥钠组给予苯巴比妥钠注射液,按80 mg·kg^(-1)·d^(-1)连续腹腔给药5 d。实验结束后采用UV法测定各组大鼠肝脏微粒体中Ⅰ相代谢酶和Ⅱ相代谢酶活性。结果:与空白对照组相比,石榴皮鞣质高、中、低剂量组均能降低细胞色素P450(CYP450)总蛋白和细胞色素b5(CYPb5)含量及抑制氨基比林N-脱甲基酶(ADM)活性,差异有统计学意义(P<0.01);石榴皮鞣质高、中剂量组能显著抑制红霉素N-脱甲基酶(ERD)活性,差异有统计学意义(P<0.01);石榴皮鞣质高剂量组能显著降低谷胱甘肽S-转移酶(GST)活性,差异有统计学意义(P<0.01)。结论:石榴皮鞣质对正常大鼠肝药酶活性具有一定抑制作用,能降低CYP3A和CYP2E1的表达,且与给药剂量有关。

Objective： To study the effect of tannins from Pericarpium Granati on hepatic microsomal enzyme in rats. Methods： Thirty Wistar rats were randomly divided into five groups ： the blank control group, high, medium and low dose groups of tannins from Pericarpium Granati, the positive control group of phenobarbital sodium. The blank control group was given physiological saline. The three different doses groups were respectively given tannins from Pericarpium Granati orally at the dose of 150,100 and 75 mg·kg^-1·d^-1 for 7 days. The positive control group was given phenobarbital sodium 80 mg·kg^-1·d^-1 with intramuscular injection for 5 days. At the end of the experiment, the activity of I and U phase metabolic enzymes in liver microsomes of each group was determined by UV. Results： Compared with the blank control group, the high, medium and low dose groups of tannins from Pericarpium Granati could significantly decrease the content of CYP 450 and CYPh5, and inhabit the activity of ADM （P 〈 0.01 ） ; the high and medium dose group could significantly inhibit ERD enzyme activity （ P 〈 0.01 ） ; the high dose group could significantly reduce GST enzyme activity （P 〈 0.01 ）. Conclusion： Tannins from Pericarpium Granati has notably inhibitory effect on hepatic microsomal enzyme in rats, which can reduce the expression of CYP3A and CYP2E1 in a dose-dependent manner.