甲亢患者IgG特异性激活补体类循环免疫复合物的研究

Study on Circulating Immune Complexes of IgG Specific Activing Complement in Patients with Graves' Disease

目的 探讨甲状腺机能亢进 (Gravesdisease,GD)患者IgG特异性激活补体类循环免疫复合物 (IgG/C3 -TCIC)的存在状况及其病理生理意义。方法 采用捕捉法ELISA以羊IgG抗人C3 为包被抗体 ,HRP -羊抗人IgG为检测抗体 ,检测了 2 19例GD患者和 10 5例健康人血清中IgG/C3 -TCIC ;按不同年龄、性别、血清Ig、C3 、T3 、T4、FT3 、FT4、TG和TM水平正常与否 ,比较其阳性率 ;并分析其与血清总、游离、复合Ig与C3 的相关性。结果 GD患者血清中IgG/C3 -TCIC含量和阳性率显著高于正常对照组 (P <0 0 1,0 0 0 1) ;其阳性率与年龄、性别及血清甲状腺激素水平无关 ,仅在血清IgA水平升高组显著高于IgA正常组 (P <0 0 5 ) ;患者IgG/C3 -TCIC仅与复合IgM呈正相关 (r=0 1732 ,P <0 0 5 ) ,而正常对照IgG/C3 -TCIC与总游离IgM及复合C3 呈正相关 (r =0 4 96 8,0 4 731,0 2 113;P<0 0 0 1,0 0 0 1,0 0 5 )。结论 GD患者存在特异性免疫功能紊乱。Ig类型转换于该类TCIC的形成具有重要的调节作用。并提出深入研究其机理 。

Objective To explore existing state and its pathology meanings of the two component circulating immune complexes of IgG specific activing complement(IgG/C\-3-TCIC) in patients with Graves' disease(GD).Methods By a capture ELISA,used coat IgG anti human C\-3 for coated antibody,HRP coat anti human IgG for second antibody,taking 219 cases of GD as a test group and 105 healthy individuals as a control one,IgG/C\-3-TCIC were characterized in this paper to compare its positive rate between the normal and abnormal levels of serum Ig,C\-3,T\-3,T\-4,FT\-3,FT\-4,TG,TM and different age,sex,and to analyse the correlation between IgG/C\-3-TCIC and serum total,free,composed Ig and C\-3.Results Both the levels and positive rates of IgG/C\-3-TCIC in GD patients were remarkably higher than those in the controls( P<0 01,0 001), its positive rates were no relations with age,sex and the levels of serum thyroxine,only notablely higher in the levels of serum IgA than those of the normal groups ( P<0 05) .IgG/C\-3-TCIC in GD patients only correlated with the complexed IgM( r=0 173 2,P<0 05) .In contrast,IgG/C\-3-TCIC correlated with total,free IgM and complexed C\-3( r=0 496 8,0 473 1,0 211 3,P<0 001,0 001,0 05) .Conclusion There were specific immune disorder in patients with Graves' disease.Ig switch had an important effect on regulating the formation of IgG/C\-3-TCIC.It cut a new approach to further illustrate the mechanism of the relational autoimmune diseases.