染料木黄酮对体外细胞增殖的抑制作用研究

Study on Antiproliferation Effects of Genistein in Vitro

目的 研究染料木黄酮的抗癌作用及其对正常细胞的毒性大小。方法 观察染料木黄酮对肺癌YTMLC -90、胃低分化腺癌SGC - 790 1、中国仓鼠卵巢CHO细胞株体外增殖和正常人外周血淋巴细胞活性和功能状态的影响。结果 染料木黄酮对正常人外周血淋巴细胞基本无抑制作用 ,而对YTMLC - 90、SGC - 790 1均有抑制作用 ,且随着剂量的增加和时间的延长 ,抑制作用也相应增强 ,其最低有效抑制浓度为 18 5 μmol/L。 结论 染料木黄酮可抑制肿瘤细胞增殖 ,可作为一种较好的防癌抗癌物质在肿瘤的治疗和预防过程中发挥重要的作用。但本研究还发现染料木黄酮对CHO细胞也有一定的抑制作用 。

Objective To elucidate the effects of genistein on cancer or normal cell.Methods The inhibitory effects of genistein on the proliferation of human gatric cancer cell line SGC-7901,human lung cancer line YTMLC-90,Chinese hamster ovary cell line CHO and peripheral blood lymphocyte from healthy person were studied.Results There are significantly inhibitory effects of genistein on proliferation of SGC-7901,YTMLC-90 and CHO cell line with a dose-effect and time-effect relationship.The lowest inhibitary concentration of genistein is 18.5μmol/L.Genistein affected the normal peripheral blood lymphocyte with a very slight extent.Conclusion Genistein inhibits the growth of tumor cells,so that it may have important significance in the treatment and prevention of cancer as a effective ingredient of health food.However,it is also found in this study that genistein was toxic to CHO cell line,and a further study is needed for the comphrehensive evaluation of biological activities of genistein.