维拉帕米和尼莫地平对硫芥中毒大鼠血清NO的影响

Effect of Verapamil and Nimodipine on Level of Nitric Oxide in Serum of Rats Poisoned by Mustard

目的 研究维拉帕米、尼莫地平这两种钙离子通道阻滞剂对硫芥中毒大鼠血清中一氧化氮水平的影响 ,并初步探讨其机制。方法 将硫芥染毒的大鼠分别用螺旋藻多糖、谷胱甘肽、维拉帕米、尼莫地平、以维拉帕米为主的复方一、以维拉帕米为主的复方二、以尼莫地平为主的复方三和以尼莫地平为主的复方四救治 ,并设立阴性对照组和阳性对照组。结果 阴性对照组的一氧化氮水平和维拉帕米、尼莫地平、复方一、复方二、复方三和复方四治疗组一致 ;阳性对照组的一氧化氮水平和谷胱甘肽治疗组一致 ;饮用PS治疗组的一氧化氮水平介于阴、阳性对照组之间。结论 维拉帕米、尼莫地平能够拮抗硫芥中毒所致的高一氧化氮水平 ,其机制可能与维拉帕米和尼莫地平阻滞Ca2 +

Objective To study the effect of verapamil and nimodipine on the level of nitric oxide (NO) in serum of the rats which were poisoned by sulfur mustard,and to inquire into the mechanism initially.Methods The rats which were poisoned by sulfur mustard were rescued differently with polysaccharide of spiral alga (PS),reduced glutathione (GSH),verapamil,nimodipine,compound No.1 which was priority to verapamil,compound No.2 which was priority to verapamil,compound No.3 which was priority to nimodipine,compound No.4 which was priority to nimodipine,and established the negative contrast group and the positive contrast group.Results The level of NO of negative contrast group was as same as those of that groups which were rescued differently with verapamil,nimodipine,compound No.1,compound No.2,compound No.3,and compound No.4.The level of NO of positive contrast group was as same as the group that was rescued with GSH.The level of NO of drinking PS group was between those of the negative contrast group and the positive contrast group.Conclusion Verapamil and nimodipine can antagonize the high level of NO induced by sulfur mustard,the mechanism maybe relate to verapamil and nimodipine inhibiting the Ca\+\{2+\} to enter cell.