江苏省艾滋病病毒感染流行病学研究

Seroepidemiological and Molecular Epidemiological Study on HIV Infecion in Jiangsu Province

目的 了解江苏省艾滋病病毒 (HIV)感染的流行特征、传染来源及危险因素 ,推断其流行趋势 ,为制定防制策略提供依据。方法 使用酶联免疫吸附法及免疫印迹试验对高危人群进行HIV抗体检测 ,采集感染者全血分离淋巴细胞用套式聚合酶链反应扩增HIV - 1DNA片段进行序列分析 ,鉴定病毒亚型。结果 从 1986年至 2 0 0 1年 6月底 ,全省共监测各类人群 95 4 4 4 5人次 ,发现HIV抗体阳性者 133例 ,检出率为 0 14‰ ,其中艾滋病病人 (AIDS) 8例 ,死亡 4例。对 19例HIV感染者进行了亚型鉴定 ,HIV - 1M群共有 4种亚型。A ,B′ ,C ,D。献血员中 85 71%为B′亚型 ,吸毒人群均为C亚型 ,回国劳务人员为A和D亚型。结论 江苏省艾滋病病毒感染的流行已进入快速增长期 ,以静脉吸毒和性途径传播为主 ,HIV - 1毒株亚型复杂 ,防治工作形势严峻。

Objective To analyze the epidemic characteristics,sources of infection,risk factors and trend of HIV infection in Jiangsu province.Methods HIV antibody was detected by ELISA and WB in the serum of high risk groups.The anticongulated blood of the infected people was collected to separate previrus DNA.Then it was amplified between C2-V3 region by nPCR.The amplified products were sequenced to identify HIV-1 subtypes.Results From January 1986 to the end of June 2001,a total number of 954 445 serum samples were detected for HIV antibody.One hundred and thirty-three people with HIV-1 sero-positive were identified,accounting for 0.14‰ of the total numbers.There were 8 patients with AIDS in which 4 cases died.Four subtypes of group M HIV-1 were found in Jiangsu:A,B′,C,D.Subtype B′ was circulating among commercial blood donors,subtype C was among IDUs,while subtype A and D were among labourers returned from abroad.Conclusion The number of HIV/AIDS cases increased rapidly in recent years in Jiangsu.The major transmission route were through IDUs and sexual contacts.HIV-1 subtypes were complex.It was necessary to conduct comprehensive STD/AIDS surveillance and behaviour intervention of high risk groups.